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Updated: Jun 2, 2026
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Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
Published on: November 1, 2017
Hematopoietic progenitors express embryonic stem cell and germ layer genes
Bernard Pessac1, Mehdi Ait Bara, David Ford
1CNRS UMR, université Paris Descartes, France. bernard.pessac@wanadoo.fr
Comptes Rendus Biologies
|April 26, 2011
Summary
Adult bone marrow contains CD34+ cells that naturally express embryonic stem cell (ESC) genes. These cells spontaneously differentiate into multiple lineages without genetic manipulation, exhibiting embryonic-like stem cell characteristics.
Area of Science:
- Stem cell biology
- Regenerative medicine
- Cellular reprogramming
Background:
- Cell therapy for tissue regeneration needs cells with high self-renewal and differentiation capacity.
- Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) possess these qualities.
- Current methods often involve genetic manipulation to achieve pluripotency.
Purpose of the Study:
- To investigate the potential of normal adult mammalian bone marrow cells for regenerative applications.
- To identify naturally occurring cells with embryonic-like stem cell properties.
- To determine if these cells express genes associated with pluripotency and multiple germ layers without genetic induction.
Main Methods:
- Analysis of gene expression in CD34+ cells isolated from adult mammalian bone marrow.
- Identification of genes characteristic of ESCs and iPSCs.
- Detection of genes associated with ectoderm, mesoderm, and endoderm lineages.
Main Results:
- Normal adult mammalian bone marrow CD34+ cells naturally express genes required for generating iPSCs.
- These CD34+ cells spontaneously express genes from all three embryonic germ layers: ectoderm, mesoderm (cardiac muscle), and endoderm (pancreatic, intestinal).
- No genetic manipulation was required for the expression of these pluripotency and differentiation-related genes.
Conclusions:
- Adult bone marrow contains naturally occurring CD34+ cells with ESC-like properties.
- These cells exhibit spontaneous pluripotency and multipotency without genetic modification.
- These findings suggest a novel source of cells for regenerative medicine and tissue engineering.
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