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Rapid HCP5 single-nucleotide polymorphism genotyping: a simple allele-specific PCR method for prediction of
Cristian A Galván1, Osvaldo C Elbarcha, Eduardo J Fernández
1Laboratorio de Análisis Clínicos Especializados (LACE), Córdoba, Argentina.
Background:
The most important factor limiting the success of an antiretroviral therapy is toxicity. The HLA-B*5701 allele is predictive of hypersensitivity reaction to Abacavir, and this gene is in a perfect linkage disequilibrium with the rs2395029 SNP present in the HCP5 gene.
Methods:
Genomic DNA was extracted from blood obtained from 201 unrelated healthy Argentinean volunteers. The DNA was subjected to an allele-specific PCR method. Sequencing was performed to validate the test results.
Results:
We were successful to amplify specific fragment of interest from the DNA samples. The method is easy, specific and reproducible.
Conclusions:
The application of this methodology is a rapid and simple method to detect the HCP5 polymorphism (rs2395029) previous to administration of Abacavir in patients with HIV infection.
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