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Updated: Jun 2, 2026
A General Method for Detecting Nitrosamide Formation in the In Vitro Metabolism of Nitrosamines by Cytochrome P450s
Published on: September 25, 2017
Detection and characterization of a multicopper oxidase from Nitrosomonas europaea
Thomas J Lawton1, Amy C Rosenzweig
1Department of Molecular Biosciences, Northwestern University, Evanston, Illinois, USA.
Abstract:
Blue copper oxidase (BCO) is a multicopper oxidase (MCO) found in Nitrosomonas europaea as well as in other ammonia-oxidizing organisms. In this chapter, we detail methods used to detect, isolate, and characterize BCO from N. europaea. A method for identifying and classifying MCOs commonly found in nitrifiers based on primary sequence is also described.
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