Related Experiment Videos
[Characterization of murine monoclonal antibodies against the common acute leukemia antigen (CALLA)]
M Seifert1, W Eichler, H Fiebig
1Bereich Tierphysiologie und Immunbiologie, Karl-Marx-Universität Leipzig.
Allergie Und Immunologie
|January 1, 1990
Abstract:
This study presents two murine monoclonal antibodies which react with the Common Acute Lymphoblastic Leukemia Antigen (CALLA). Both antibodies can be used for the diagnosis of common ALL (cALL). Indirect immunofluorescence studies (FACS-analysis) showed that the antibodies react with granulocytes and different human cell lines (Nalm-6, Reh, Raji, CCRF-CEM). The monoclonal antibodies BL-CALLA/1 and BL-CALLA/2 identify a single polypeptide chain of 95 kD. Both antibodies recognize the same or closely related epitope of the CALLA-molecule and are able to modulate in vitro the antigen on the CALLA-positive cell line Reh.