Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Spin–Spin Coupling: Two-Bond Coupling (Geminal Coupling)01:20

Spin–Spin Coupling: Two-Bond Coupling (Geminal Coupling)

Two NMR-active nuclei bonded to a central atom can be involved in geminal or two-bond coupling. Geminal coupling is commonly seen between diastereotopic protons in chiral molecules and unsymmetrical alkenes, among others.
The central atom need not be NMR-active because its electrons are affected by the electron polarization of the spin-active atoms. However, spin information is transmitted less effectively than in one-bond coupling, and 2J values are usually weaker than 1J values. The energy of...
Spin–Spin Coupling: Three-Bond Coupling (Vicinal Coupling)01:22

Spin–Spin Coupling: Three-Bond Coupling (Vicinal Coupling)

Vicinal or three-bond coupling is commonly observed between protons attached to adjacent carbons. Here, nuclear spin information is primarily transferred via electron spin interactions between adjacent C‑H bond orbitals. This generally favors the antiparallel arrangement of spins, so 3J values are usually positive.
The extent of coupling depends on the C‑C bond length, the two H‑C‑C angles, any electron-withdrawing substituents, and the dihedral angle between the involved orbitals. The...
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
Spin–Spin Coupling: One-Bond Coupling01:17

Spin–Spin Coupling: One-Bond Coupling

Coupling interactions are strongest between NMR-active nuclei bonded to each other, where spin information can be transmitted directly through the pair of bonding electrons. While nuclei polarize their electrons to the opposite spins, the bonding electron pair has opposite spins. Configurations with antiparallel nuclear spins are expected to be lower in energy. When coupling makes antiparallel states more favorable, J is considered to have a positive value. The one-bond coupling constant, 1J,...
Induced Electric Dipoles01:28

Induced Electric Dipoles

A permanent electric dipole orients itself along an external electric field. This rotation can be quantified by defining the potential energy because the external torque does work in rotating it. Then, the potential energy is minimum at the parallel configuration and maximum at the antiparallel configuration. While the former is a stable equilibrium, the latter is an unstable equilibrium.
Since the absolute value of potential energy holds no physical meaning, its zero value can be chosen as per...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Lipid-Conjugation Strategy for Intracellular Reactive Oxygen Species Control in Hepatic Cells.

Angewandte Chemie (International ed. in English)·2026
Same author

Accordion-like tuning of composite pulse dipolar recoupling in solid-state NMR.

The Journal of chemical physics·2026
Same author

Longitudinal Pulsed Dynamic Nuclear Polarization Transfer via Periodic Optimal Control.

The journal of physical chemistry letters·2026
Same author

Selective Mineral Recovery from Seawater by Ion-Exchangeable Metal-Organic Framework Glasses.

Journal of the American Chemical Society·2026
Same author

Nonporous hydrophobic organic crystals for carbon dioxide capture via chain-melting phase transition.

Nature communications·2026
Same author

Ultrasound Use in Anesthesiology and Intensive Care in the Nordic Countries-An International Survey on Availability, Frequency of Use, Operator Training, and Assessment.

Acta anaesthesiologica Scandinavica·2026
Same journal

Chemoselectivity and stereoselectivity have been key factors in the development of fine organic synthesis. Introduction.

Topics in current chemistry·2016
Same journal

Hypervalent Iodine-Induced Oxidative Couplings (New Metal-Free Coupling Advances and Their Applications in Natural Product Syntheses).

Topics in current chemistry·2016
Same journal

Halogen Bonding in Hypervalent Iodine Compounds.

Topics in current chemistry·2016
Same journal

Phenol Dearomatization with Hypervalent Iodine Reagents.

Topics in current chemistry·2016
Same journal

Preface.

Topics in current chemistry·2016
Same journal

Preface: solar energy for fuels.

Topics in current chemistry·2016
See all related articles

Related Experiment Video

Updated: Jun 2, 2026

Recombination Dynamics in Thin-film Photovoltaic Materials via Time-resolved Microwave Conductivity
11:30

Recombination Dynamics in Thin-film Photovoltaic Materials via Time-resolved Microwave Conductivity

Published on: March 6, 2017

Dipolar recoupling.

Niels Chr Nielsen1, Lasse A Strassø, Anders B Nielsen

  • 1Department of Chemistry, Aarhus University, Aarhus C, 8000, Aarhus, Denmark. ncn@chem.au.dk

Topics in Current Chemistry
|April 26, 2011
PubMed
Summary
This summary is machine-generated.

Dipolar recoupling in solid-state NMR spectroscopy provides crucial insights into molecular structure and spin dynamics. This technique forms the foundation for advanced multidimensional NMR experiments, enhancing structural and dynamic analysis in biological systems.

More Related Videos

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
10:54

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

Published on: February 23, 2016

Retropinacol/Cross-pinacol Coupling Reactions - A Catalytic Access to 1,2-Unsymmetrical Diols
10:12

Retropinacol/Cross-pinacol Coupling Reactions - A Catalytic Access to 1,2-Unsymmetrical Diols

Published on: April 4, 2014

Related Experiment Videos

Last Updated: Jun 2, 2026

Recombination Dynamics in Thin-film Photovoltaic Materials via Time-resolved Microwave Conductivity
11:30

Recombination Dynamics in Thin-film Photovoltaic Materials via Time-resolved Microwave Conductivity

Published on: March 6, 2017

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
10:54

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

Published on: February 23, 2016

Retropinacol/Cross-pinacol Coupling Reactions - A Catalytic Access to 1,2-Unsymmetrical Diols
10:12

Retropinacol/Cross-pinacol Coupling Reactions - A Catalytic Access to 1,2-Unsymmetrical Diols

Published on: April 4, 2014

Area of Science:

  • Solid-state Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Biophysical Chemistry
  • Structural Biology

Background:

  • Dipolar recoupling is a fundamental technique in solid-state NMR.
  • It enables the measurement of distances and interactions between nuclear spins.
  • Essential for extracting detailed structural and dynamic information.

Purpose of the Study:

  • To elucidate the principles and applications of dipolar recoupling in solid-state NMR.
  • To provide a framework for understanding multidimensional NMR experiments based on dipolar recoupling.
  • To showcase the development and application of dipolar recoupling methods in biological solid-state NMR.

Main Methods:

  • Introduction to the basic formalism of dipolar recoupling.
  • Presentation of design principles for effective recoupling sequences.
  • Description of a series of homo- and heteronuclear dipolar recoupling experiments.

Main Results:

  • Demonstration of how dipolar recoupling accesses structural information.
  • Illustration of magnetization transfer between spins using dipolar recoupling.
  • Highlighting the evolution of advanced dipolar recoupling techniques.

Conclusions:

  • Dipolar recoupling is indispensable for multidimensional solid-state NMR.
  • The described methods facilitate detailed structural and dynamic analysis.
  • Applications in biological solid-state NMR are significantly advanced by these techniques.