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Related Concept Videos

Cell Lines01:16

Cell Lines

A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...
Stem Cell Culture01:17

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
Cell Culture01:21

Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media

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Related Experiment Video

Updated: Jun 2, 2026

Freezing, Thawing, and Packaging Cells for Transport
07:32

Freezing, Thawing, and Packaging Cells for Transport

Published on: July 2, 2008

Storage of cell lines.

Katharine A Parker1

  • 1Translational Oncology Research Centre, Queen Alexandra Hospital, Portsmouth, UK. iancreephd@gmail.com

Methods in Molecular Biology (Clifton, N.J.)
|April 26, 2011
PubMed
Summary

Proper cell line storage is crucial for research. Key factors include cell health, operator skill, record-keeping, monitoring, aseptic technique, and reagent quality for successful cryopreservation and propagation.

Area of Science:

  • Cell Biology
  • Cryobiology
  • Laboratory Management

Background:

  • Cell line storage is fundamental for reproducible biological research.
  • Improper cell line cryopreservation can lead to significant research setbacks and resource loss.
  • Maintaining cell line integrity is essential for downstream experimental validity.

Purpose of the Study:

  • To highlight critical factors for successful cell line storage.
  • To emphasize the importance of meticulous laboratory practices in cell line preservation.
  • To guide researchers in optimizing cell line cryopreservation and subsequent propagation.

Main Methods:

  • Review of best practices in cell line management.
  • Emphasis on operator experience and aseptic techniques.

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Thawing, Culturing, and Cryopreserving Drosophila Cell Lines
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Thawing, Culturing, and Cryopreserving Drosophila Cell Lines

Published on: April 16, 2019

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Last Updated: Jun 2, 2026

Freezing, Thawing, and Packaging Cells for Transport
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Freezing, Thawing, and Packaging Cells for Transport

Published on: July 2, 2008

Thawing, Culturing, and Cryopreserving Drosophila Cell Lines
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Thawing, Culturing, and Cryopreserving Drosophila Cell Lines

Published on: April 16, 2019

  • Discussion of reagent quality and record-keeping importance.

    • Main Results:

    • Successful cell line storage is contingent upon multiple variables.
    • Poorly managed cell lines (unhealthy, contaminated, mislabeled) pose significant risks.
    • Adherence to stringent protocols ensures cell line viability and usability.

    Conclusions:

    • Meticulous record-keeping, vigilant monitoring, and aseptic techniques are paramount for successful cell line storage.
    • High-quality reagents and operator expertise are vital for cryopreservation and downstream cell propagation.
    • Implementing these practices safeguards research integrity and resource investment.