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Related Experiment Video

Updated: Jun 2, 2026

Real-time Cytotoxicity Assays in Human Whole Blood
08:27

Real-time Cytotoxicity Assays in Human Whole Blood

Published on: November 7, 2014

Real-time cytotoxicity assays.

Donald Wlodkowic1, Shannon Faley, Zbigniew Darzynkiewicz

  • 1The BioMEMS Research Group, Department of Chemistry, University of Auckland, Auckland, New Zealand. d.wlodkowic@elec.gla.ac.uk

Methods in Molecular Biology (Clifton, N.J.)
|April 26, 2011
PubMed
Summary
This summary is machine-generated.

This study introduces new real-time cell death assays using propidium iodide (PI) and SYTOX Green probes. These innovative methods enable kinetic analysis for accelerated anticancer drug discovery and predictive toxicology.

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Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells
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Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells

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Published on: November 7, 2014

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Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells
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Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells

Published on: February 2, 2013

Area of Science:

  • Biotechnology and Biomedical Assays
  • Cancer Therapeutics and Drug Discovery
  • Cellular Signaling and Toxicology

Background:

  • Advancing therapeutic target validation requires assays probing spatial and temporal signaling network dynamics.
  • Current cell death assays for anticancer drug profiling are often endpoint measurements, lacking kinetic insights into stochastic processes like tumor cell death.

Purpose of the Study:

  • To develop innovative protocols for real-time kinetic analysis of tumor cell death.
  • To provide methods adaptable for both flow cytometry and time-lapse fluorescence imaging.
  • To facilitate accelerated anticancer drug discovery and high-throughput screening.

Main Methods:

  • Development of novel real-time cell death analysis protocols.
  • Utilized propidium iodide (PI) and SYTOX Green fluorescent probes.
  • Adaptable protocols for flow cytometry and time-lapse fluorescence imaging.

Main Results:

  • Established innovative protocols for real-time kinetic analysis of tumor cell death.
  • Demonstrated adaptability of these assays to flow cytometry and time-lapse fluorescence imaging.
  • Highlighted potential for significant time savings and kinetic data acquisition.

Conclusions:

  • The developed real-time cell death assays offer a significant advancement over endpoint methods.
  • These assays provide kinetic data crucial for understanding stochastic cell death processes.
  • The protocols have strong potential for application in accelerated anticancer drug discovery and predictive toxicology screening.