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Updated: Jun 2, 2026
08:31
Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells
Published on: September 16, 2014
Enantioselective sensing by luminescence.
Alessandro Accetta1, Roberto Corradini, Rosangela Marchelli
1Dipartimento di Chimica Organica e Industriale, Università di Parma, Parco Area delle Scienze 17/A, 43124, Parma, Italy. alessandro.accetta@unipr.it
Topics in Current Chemistry
|April 26, 2011
Summary
Luminescent sensors offer advanced enantiomeric analysis for chiral molecules, crucial for pharmaceuticals and agrochemicals. These sensors utilize various mechanisms for precise optical purity assessment and fast screening.
Area of Science:
- Analytical Chemistry
- Sensor Technology
- Chiral Analysis
Background:
- Enantiomeric purity is critical in pharmaceutical and agrochemical industries.
- Luminescent sensors provide spectroscopic methods for assessing optical purity.
- Chemosensors are developed for analyzing both luminescent and non-luminescent chiral compounds.
Purpose of the Study:
- To review recent advancements in chiral molecule sensing using luminescent systems.
- To outline diverse sensing mechanisms for enantiomeric analysis.
- To highlight techniques enabling high enantioselectivity and rapid screening.
Main Methods:
- Fluorescence quenching (metal complexes, photoinduced electron transfer - PET)
- Fluorescence enhancement via PET inhibition
- Analyte-induced conformational changes
- Modulation of excimer/exciplex formation
- Aggregation-induced emission enhancement (AIEE)
- Advanced techniques: anisotropy, gated detection, circularly polarized luminescence (CPL)
Main Results:
- Demonstration of various luminescence-based mechanisms for chiral sensing.
- Successful application of techniques like CPL for enantiomeric discrimination.
- Identification of methods offering high enantioselectivity and suitability for high-throughput screening.
Conclusions:
- Luminescent sensors represent a powerful tool for enantiomeric analysis.
- Diverse sensing mechanisms and advanced techniques are expanding capabilities in chiral recognition.
- The field shows promise for developing rapid and highly selective methods for chiral compound analysis.
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