Related Experiment Video
Updated: Jun 2, 2026

CN-GELFrEE - Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis
Published on: February 29, 2016
[Native electrophoresis in cell proteomics: BN-PAGE and CN-PAGE]
Abstract:
The presented mini-review aims to attract the attention of domestic researchers for rapid, cheap and easily reproducible method of native polyacrylamide gel electrophoresis (PAGE), which for some reason has not yet found application in our country. The review collected the most interesting examples of the use of three types of native electrophoresis (BN-PAGE, CN-PAGE and hrCN-PAGE) to study the peculiarities of proteomes of various animal, plant and bacterial cells. The references to fundamental reviews, basic protocols, modifications of the initial methods and the examples of the combination of native electrophoresis with other chemical or physical methods are presented. Particular attention to the principles of BN-, CN- and hrCN-PAGE as well as to their advantages and disadvantages is paid.
More Related Videos
Related Concept Videos
SDS-PAGE
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
Two-dimensional Gel Electrophoresis
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...
Electrophoresis: Overview
There...
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

