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Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors
Published on: July 8, 2012
Plasticity and maintenance of hematopoietic stem cells during development
Suman Kanji1, Vincent J Pompili, Hiranmoy Das
1Cardiovascular Stem Cell Research Laboratories, The Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, Ohio 43210, USA.
Recent Patents on Biotechnology
|April 27, 2011
Summary
Hematopoietic stem cells (HSCs) self-renewal and differentiation are balanced by developmental signals. Understanding these signals is crucial for expanding HSCs ex vivo for clinical applications.
Area of Science:
- Developmental Biology
- Hematology
- Stem Cell Biology
Background:
- Hematopoietic stem cells (HSCs) maintain blood cell production through a balance of self-renewal and differentiation.
- HSCs originate during embryonic development, migrating through various anatomical sites.
- Both intrinsic transcriptional programs and extrinsic niche signals regulate HSC fate.
Purpose of the Study:
- To review the developmental origins and regulation of HSCs.
- To focus on signals that induce the adult HSC program.
- To highlight information critical for ex vivo HSC expansion and clinical applications.
Main Methods:
- Literature review of developmental signals.
- Analysis of HSC regulation during embryonic development.
- Examination of clinical applications and patents related to HSCs.
Main Results:
- HSCs differentiate into mature blood cells under physiological conditions.
- Under pathological conditions, HSCs can transdifferentiate into non-hematopoietic cells.
- This transdifferentiation challenges the traditional hierarchical model of hematopoiesis.
Conclusions:
- Knowledge of developmental signals is key for ex vivo HSC expansion.
- HSCs possess a plasticity that extends beyond the hematopoietic system.
- This review provides insights into clinical applications and patents involving HSCs.
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