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Updated: Jun 2, 2026

Physical, Chemical and Biological Characterization of Six Biochars Produced for the Remediation of Contaminated Sites
Published on: November 28, 2014
Application of the knowledge-based approach to strain selection for a bioaugmentation process of phenanthrene- and
A Ibarrolaza1, B M Coppotelli, M T Del Panno
1Centro de Investigación y Desarrollo en Fermentaciones Industriales, CINDEFI (UNLP; CCT-La Plata, CONICET), La Plata, Argentina. guri@biol.unlp.edu.ar
Aims:
The objective of this study was to apply the knowledge-based approach to the selection of an inoculum to be used in bioaugmentation processes to facilitate phenanthrene degradation in phenanthrene- and Cr(VI)-co-contaminated soils.
Methods And Results:
The bacterial community composition of phenanthrene and phenanthrene- and Cr(VI)-co-contaminated microcosms, determined by denaturing gradient gel electrophoresis analysis, showed that members of the Sphingomonadaceae family were the predominant micro-organisms. However, the Cr(VI) contamination produced a selective change of predominant Sphingomonas species, and in co-contaminated soil microcosms, a population closely related to Sphingomonas paucimobilis was naturally selected. The bioaugmentation process was carried out using the phenanthrene-degrading strain S. paucimobilis 20006FA, isolated and characterized in our laboratory. Although the strain showed a low Cr(VI) resistance (0·250 mmol l⁻¹); in liquid culture, it was capable of reducing chromate and degrading phenanthrene simultaneously.
Conclusion:
The inoculation of this strain managed to moderate the effect of the presence of Cr(VI), increasing the biological activity and phenanthrene degradation rate in co-contaminated microcosm.
Significance And Impact Of The Study:
In this study, we have applied a novel approach to the selection of the adequate inoculum to enhance the phenanthrene degradation in phenanthrene- and Cr(VI)-co-contaminated soils.
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