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[Effects of oleanolic acid on apoptosis and PTEN expression of Jurkat cells]
1Diagnostic and Therapeutic Center of Hematologic Diseases, China Medical University, Shenyang, Liaoning Province, China.
Abstract:
This study was aimed to explore the effects of oleanolic acid on PTEN expression and apoptosis of Jurkat cells. The inhibitory rate was measured by Cell Counting Kit-8. The apoptotic nucleus morphous was observed by Hoechst 33258 staining. The apoptosis rate of Jurkat cells were determined by flow cytometry with Annexin V/PI double staining. PTEN mRNA and protein were detected by quantitative real-time PCR and Western blot respectively. The results showed that oleanolic acid inhibited the proliferation of Jurkat cells in time- and dose-dependent manners. The 50% growth inhibition (IC(50)) at 12, 24 and 48 hours were about 85.35 µmol/L, 53.66 µmol/L and 33.18 µmol/L respectively. Flow cytometric assay showed that the apoptotic rates of Jurkat cells treated with oleanolic acid (0, 40, 80 and 160 µmol/L) for 24 hours were 6.72%, 19.8%, 28.72% and 30.12% (p < 0.05). PTEN mRNA and protein expressions were up-regulated in Jurkat cells treated with oleanolic acid of concentration 80 µmol/L and 160 µmol/L for 24 hours. It is concluded that up-regulation of PTEN mRNA and PTEN protein may be involved in oleanolic acid-induced Jurkat cell apoptosis.
Insights
Oleanolic acid effectively inhibits Jurkat cell proliferation and induces apoptosis in a dose- and time-dependent manner. This effect is linked to the increased expression of PTEN (Phosphatase and tensin homolog).
Area of Science:
- Molecular Biology
- Cell Biology
- Pharmacology
Context:
- Jurkat cells are a human T lymphocyte cell line commonly used in leukemia research.
- Oleanolic acid is a naturally occurring pentacyclic triterpenoid with potential therapeutic properties.
- Understanding the mechanisms of action of natural compounds like oleanolic acid is crucial for drug discovery.
Purpose:
- To investigate the impact of oleanolic acid on the proliferation and apoptosis of Jurkat cells.
- To examine the effect of oleanolic acid on the expression of PTEN (Phosphatase and tensin homolog) mRNA and protein.
- To elucidate the role of PTEN in oleanolic acid-induced apoptosis.
Summary:
- Oleanolic acid demonstrated a time- and dose-dependent inhibition of Jurkat cell proliferation, with calculated IC(50) values decreasing over time.
- Apoptosis rates in Jurkat cells significantly increased following treatment with oleanolic acid, as evidenced by flow cytometry and Hoechst staining.
- Quantitative real-time PCR and Western blot analyses revealed a significant up-regulation of both PTEN mRNA and protein in response to oleanolic acid treatment.
Impact:
- The findings suggest that oleanolic acid possesses anti-proliferative and pro-apoptotic properties against Jurkat cells.
- The observed up-regulation of PTEN indicates its potential involvement as a key mediator in the apoptotic pathway induced by oleanolic acid.
- This study provides a foundation for exploring oleanolic acid as a potential therapeutic agent in T-cell leukemia, highlighting PTEN as a critical molecular target.
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