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Related Experiment Video

Updated: Jun 2, 2026

Investigating the Function of Coronin A in the Early Starvation Response of Dictyostelium discoideum by Aggregation Assays
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Human corin isoforms with different cytoplasmic tails that alter cell surface targeting.

Xiaofei Qi1, Jingjing Jiang, Mingqing Zhu

  • 1Cyrus Tang Hematology Center, Jiangsu Institute of Hematology, First Affiliated Hospital, Soochow University, Suzhou 215123, China.

The Journal of Biological Chemistry
|April 27, 2011
PubMed
Summary

The corin cytoplasmic tail

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Last Updated: Jun 2, 2026

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cardiology

Background:

  • Corin is a cardiac serine protease crucial for activating natriuretic peptides.
  • Its structure includes a transmembrane domain anchoring it to cardiomyocytes, but the cytoplasmic tail's function was unknown.

Purpose of the Study:

  • To investigate the role of the corin cytoplasmic tail in protein function.
  • To identify specific sequences within the tail that influence corin activity and localization.

Main Methods:

  • Comparative analysis of human and mouse corin gene sequences.
  • Expression studies in HEK 293 cells and HL-1 cardiomyocytes.
  • Cell surface labeling, glycosidase digestion, Western blotting, flow cytometry, and mutagenesis.

Main Results:

  • Identified alternative corin cytoplasmic tails in human isoforms E1 (45 amino acids) and E1a (15 amino acids).
  • Isoform E1 demonstrated higher natriuretic peptide processing activity and more efficient cell surface targeting than E1a.
  • A DDNN motif in the E1 cytoplasmic tail was found to promote cell surface targeting and activation.

Conclusions:

  • The corin cytoplasmic tail sequence significantly impacts cell surface targeting and zymogen activation.
  • Alternative splicing of corin exons generates isoforms with distinct functional properties.
  • The DDNN motif is critical for efficient corin localization and activation.