Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cross-reactivity00:42

Cross-reactivity

33.2K
Overview
33.2K
Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

8.6K
Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
8.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reciprocal signaling-metabolic crosstalk between fibroblasts and tumor cells drives cetuximab tolerance in head and neck cancers.

Journal of experimental & clinical cancer research : CR·2026
Same author

Towards a Strengthened Addictions Neuroclinical Assessment (ANA) Framework: A Perspective on Current Assessment Approaches and The Added Value of Recovery-Inspired Dimensions.

Journal of studies on alcohol and drugs·2026
Same author

Exploring Hydroxytyrosol as a Promising Virucidal Agent: In Silico and In Vitro Insights into Enveloped Viruses.

Current issues in molecular biology·2026
Same author

Multi-Patient Vision Transformer for Markerless Tumor Motion Forecasting.

Biomedicines·2026
Same author

Machine learning-based combination of the central vein sign, cortical lesions and paramagnetic rim lesions: a web-based tool for the diagnosis of multiple sclerosis.

Brain communications·2026
Same author

Analytical Performance of Nanobody-Based Immunoassay and Immunosensing Platforms for Bacteria and Toxin Detection: A Systematic Review.

Antibodies (Basel, Switzerland)·2026

Related Experiment Video

Updated: Feb 24, 2026

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
08:09

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

10.1K

Identification of relevant properties for epitopes detection using a regression model.

Jérôme Ambroise1, Joachim Giard, Jean-Luc Gala

  • 1Institute of Information and Communication Technologies, Electronics and Applied Mathematics, Université catholique de Louvain, Place du Levant 2, Louvain-Neuve 1348, Belgium. jerome.ambroise@uclouvain.be

IEEE/ACM Transactions on Computational Biology and Bioinformatics
|April 27, 2011
PubMed
Summary

This study identifies key properties to pinpoint B-cell epitopes on antigens. Geometric and conservation features, along with glycine proportion, improve epitope prediction accuracy for immunodetection applications.

More Related Videos

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
07:59

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

15.7K
Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells
13:58

Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells

Published on: October 22, 2012

18.7K

Related Experiment Videos

Last Updated: Feb 24, 2026

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
08:09

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

10.1K
A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
07:59

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

15.7K
Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells
13:58

Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells

Published on: October 22, 2012

18.7K

Area of Science:

  • Immunology
  • Computational Biology
  • Bioinformatics

Background:

  • B-cell epitopes are crucial antigen regions recognized by antibodies.
  • Accurate detection of B-cell epitopes is vital for immunodetection and immunotherapeutics.
  • Identifying epitope locations on protein surfaces remains a challenge.

Purpose of the Study:

  • To identify and evaluate properties that distinguish B-cell epitopes from non-epitope regions on antigen surfaces.
  • To develop a computational method for predicting B-cell epitope locations.

Main Methods:

  • Utilized two evaluation approaches to identify relevant properties for epitope localization.
  • Selected geometric, conservation, and chemical (glycine proportion) properties.
  • Developed a patch-based epitope localization method employing a Single-Layer Perceptron for regression.

Main Results:

  • Geometric properties were found to be the most discriminative for epitope localization.
  • Conservation scores and glycine proportion also contributed significantly to prediction accuracy.
  • The developed method generated a probability map on the antigen surface.

Conclusions:

  • The study successfully identified key features for B-cell epitope prediction.
  • The proposed method demonstrates predictive performance, validated through cross-validation and an independent test set.
  • This approach can enhance the design of immunotherapies and diagnostic tools.