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Ozonized oils: a qualitative and quantitative analysis.

Adriana Simionatto Guinesi¹, Carolina Andolfatto, Idomeo Bonetti Filho

¹Discipline of Endodontics, Department of Restorative Dentistry, Araraquara Dental School, Univ. Estadual Paulista, SP, Brazil.

Brazilian Dental Journal

|April 27, 2011

Summary

Ozonized oils used in endodontics may not contain ozone. Formaldehyde, a byproduct of ozonation, appears to be responsible for the observed therapeutic effects, not ozone itself.

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Area of Science:

Dentistry
Biochemistry
Material Science

Background:

Bacterial infections are a primary cause of endodontic problems.
There is a need for effective intracanal medicaments beyond traditional options.
Ozone exhibits potential therapeutic properties like bactericidal action and enhanced healing.

Purpose of the Study:

To chemically verify the presence of ozone in various oils and propylene glycol after ozonation.
To identify byproducts of the ozonation process, specifically formaldehyde.
To determine the actual active agent responsible for the therapeutic effects of ozonated oils.

Main Methods:

Ozonation of sunflower, castor, olive, and almond oils, plus propylene glycol.
Analysis for ozone presence using the indigo reaction test.
Analysis for formaldehyde using the chromotropic acid test.

Main Results:

Ozone was completely absent in all tested ozonated samples.
Formaldehyde was detected in all tested ozonated samples.
The therapeutic effects are likely due to formaldehyde, not ozone.

Conclusions:

Ozonated oils lack detectable ozone.
The antimicrobial and healing properties of these preparations are likely attributable to formaldehyde.
Rethinking the use of ozone in endodontics is warranted, focusing on formaldehyde's role.