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Related Experiment Videos

OCSBF-1, a maize ocs enhancer binding factor: isolation and expression during development.

K Singh1, E S Dennis, J G Ellis

  • 1Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, Canberra, Australia.

The Plant Cell
|September 1, 1990
PubMed
Summary
This summary is machine-generated.

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Researchers identified OCSBF-1, a maize protein binding to ocs-elements in plant gene promoters. This transcription factor shows differential expression in developing leaves and shares DNA-binding motifs with known factors.

Area of Science:

  • Plant Molecular Biology
  • Transcription Factor Research
  • Agrobacterium-mediated Gene Transfer

Background:

  • Ocs-elements are key DNA sequences in plant gene promoters, crucial for Agrobacterium-mediated gene transfer and viral DNA infections.
  • Understanding the proteins that interact with these elements is vital for plant biotechnology and gene regulation studies.

Purpose of the Study:

  • To isolate and characterize a maize protein that specifically binds to ocs-element DNA sequences.
  • To investigate the expression patterns and DNA-binding properties of the identified protein.

Main Methods:

  • Isolation and characterization of a maize cDNA encoding the ocs-element binding factor (OCSBF-1).
  • Analysis of OCSBF-1 gene expression in different maize tissues and developmental stages using mRNA analysis.

Related Experiment Videos

  • In vitro DNA-binding assays using truncated OCSBF-1 protein to identify functional domains.

    • Main Results:

    • A maize cDNA encoding OCSBF-1, a 21 kDa protein, was isolated. OCSBF-1 binds specifically to ocs-elements.
    • The OCSBF-1 gene is intron-less and differentially expressed, with highest transcript levels in basal portions of developing leaves, roots, and young shoots.
    • OCSBF-1 possesses a basic region and leucine zipper motif, characteristic of DNA-binding transcription factors, and binds to AP-1 and CREB sites.

    Conclusions:

    • OCSBF-1 is a novel plant transcription factor that binds to ocs-elements, suggesting its role in regulating genes involved in plant defense or development.
    • The differential expression pattern indicates tissue-specific functions for OCSBF-1 during maize development.
    • The conserved DNA-binding domain suggests functional similarities with animal transcription factors.