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Updated: Jun 2, 2026

Mouse Round Spermatid Injection

Mouse Round Spermatid Injection

Published on: January 26, 2024

Robotic ICSI (intracytoplasmic sperm injection).

Zhe Lu¹, Xuping Zhang, Clement Leung

¹Advanced Micro and Nanosystems Laboratory, University of Toronto, 5 King’s College Road, Toronto, ON M5S 3G8 Canada. zhe.lu@utoronto.ca

IEEE Transactions on Bio-Medical Engineering

|April 28, 2011

Summary

This summary is machine-generated.

This study introduces robotic intracytoplasmic sperm injection (ICSI), a novel automation for fertility treatments. The system achieves high success and survival rates, reducing human error and standardizing procedures.

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Area of Science:

Reproductive medicine
Robotics
Biotechnology

Background:

Intracytoplasmic sperm injection (ICSI) is a vital assisted reproductive technology used globally.
Manual ICSI procedures exhibit significant variability in success rates due to operator dependency and inconsistency.
Standardization of ICSI is crucial for improving reproductive outcomes.

Purpose of the Study:

To develop and evaluate a robotic system for performing intracytoplasmic sperm injection (ICSI).
To enhance the reproducibility and consistency of ICSI procedures through automation.
To reduce the dependency on highly skilled human operators in clinical ICSI.

Main Methods:

Development of a robotic ICSI system incorporating a cell holding device, advanced motion control, and computer vision algorithms.
Implementation of automated sperm handling: visual tracking, immobilization, picoliter-volume aspiration, and oocyte injection.
Utilizing a hamster oocyte-human sperm model for preliminary system validation.

Main Results:

The robotic ICSI system demonstrated high reproducibility and minimal human intervention.
Preliminary trials achieved a 90.0% success rate for robotic ICSI.
A 90.7% survival rate was observed in the hamster oocyte-human sperm model (n=120).

Conclusions:

Robotic ICSI offers a standardized, reproducible, and operator-independent alternative to manual procedures.
The developed system shows significant promise for improving the efficiency and success of fertility treatments.
Automation in ICSI can lead to more consistent and successful outcomes in assisted reproduction.