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Updated: Oct 15, 2026

Expression Analysis of Mammalian Linker-histone Subtypes
14:40

Expression Analysis of Mammalian Linker-histone Subtypes

Published on: March 19, 2012

One-step fractionation method for isolating H1 histones from chromatin under nondenaturing conditions

M Garcia-Ramirez1, S H Leuba, J Ausio

  • 1Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331-6503.

Protein Expression and Purification
|September 1, 1990
PubMed

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Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...