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Related Concept Videos

Centrifugation01:05

Centrifugation

Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
Subcellular Fractionation01:32

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
Downstream Processing01:29

Downstream Processing

Downstream processing begins once fermentation is complete and involves a series of steps to recover and purify products such as acids, vitamins, antibiotics, or proteins.Cell HarvestingFor example, for intracellular protein-based products, the first step is harvesting the cells. This is typically achieved using centrifugation or filtration to separate the cells from the liquid phase.Cell Disruption for Intracellular ProductsIf the target product is intracellular, the harvested cells must be...

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Related Experiment Video

Updated: Jun 2, 2026

Nitrogen Cavitation and Differential Centrifugation Allows for Monitoring the Distribution of Peripheral Membrane Proteins in Cultured Cells
08:24

Nitrogen Cavitation and Differential Centrifugation Allows for Monitoring the Distribution of Peripheral Membrane Proteins in Cultured Cells

Published on: August 18, 2017

Supernatant decanting on a centrifugal platform.

Chih-Hsin Shih, Chien-Hsing Lu, Wei-Li Yuan

    Biomicrofluidics
    |April 28, 2011
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a new method for decanting supernatant using centrifugal pressure and lid deformation, enabling precise liquid transfer at lower speeds. This innovation offers a cost-effective and stable solution for sample preparation in microfluidic devices.

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    Area of Science:

    • Biomedical Engineering
    • Microfluidics
    • Analytical Chemistry

    Background:

    • Accurate liquid handling is crucial for microfluidic assays.
    • Traditional supernatant decanting methods can be complex or require specific surface treatments.

    Purpose of the Study:

    • To develop a novel, cost-effective method for precise supernatant decanting on a centrifugal platform.
    • To investigate the influence of centrifugal pressure and lid deformation on decanting volume and ratio.

    Main Methods:

    • Utilizing centrifugal pressure and elastic deformation of plastic lids within a decanting chamber.
    • Manipulating rotational speed and chamber design to control liquid transfer.
    • Applying the method to on-disk plasma separation from whole blood.

    Main Results:

    • Achieved fixed-volume supernatant decanting at lower rotational speeds.
    • Demonstrated that decanted volume is controllable via deformation volume and operating parameters.
    • Showcased good stability and repeatability in on-disk plasma separation applications.

    Conclusions:

    • The novel decanting approach is simple, low-cost, and requires no surface modification.
    • This method provides a stable and repeatable solution for automated liquid handling in centrifugal microfluidic systems.
    • The technique is suitable for applications like blood-plasma separation, enhancing diagnostic platforms.