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Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance
Published on: January 3, 2012
Antibody characterization and immunoassays for palytoxin using an SPR biosensor
Betsy Jean Yakes1, Stacey L DeGrasse, Mark Poli
1Center for Food Safety and Applied Nutrition, US Food and Drug Administration, College Park, MD 20740, USA. betsy.yakes@fda.hhs.gov
Analytical and Bioanalytical Chemistry
|April 28, 2011
Summary
A new surface plasmon resonance (SPR) biosensor assay enables sensitive detection of palytoxin (PLTX), a dangerous marine toxin. This method allows for rapid quantification of PLTX in seafood, addressing a critical need for validated detection techniques.
Area of Science:
- Marine Toxinology
- Analytical Chemistry
- Biosensor Technology
Background:
- Palytoxin (PLTX) is a highly potent marine toxin causing severe human health issues through seafood contamination and direct exposure.
- Existing analytical methods for PLTX are not validated, hindering accurate detection and association with seafood-borne illnesses.
- The co-occurrence of PLTX with other marine toxins complicates diagnosis and risk assessment.
Purpose of the Study:
- To develop and validate a sensitive, rapid, and selective analytical method for quantitative palytoxin detection.
- To characterize a monoclonal anti-PLTX antibody using surface plasmon resonance (SPR) technology.
- To assess the applicability of the developed SPR assay for PLTX quantification in seafood matrices.
Main Methods:
- Development of a surface plasmon resonance (SPR) biosensor assay.
- Characterization of a monoclonal anti-PLTX antibody kinetics using an anti-mouse substrate.
- Optimization of assay parameters including buffer type, flow rate, binding time, and regeneration.
- Evaluation of assay cross-reactivity with other common seafood toxins.
- Validation of the assay in buffer and seafood matrices (grouper, clam).
Main Results:
- A sensitive and selective SPR biosensor assay for palytoxin (PLTX) was successfully developed.
- The assay demonstrated the capability to measure low- to sub-ng/mL levels of PLTX.
- Optimized assay showed minimal cross-reactivity with other seafood toxins.
- The SPR assay accurately quantified PLTX in both buffer and seafood samples.
Conclusions:
- The developed SPR biosensor provides a robust and rapid method for quantitative PLTX detection.
- This assay facilitates accurate risk assessment of PLTX contamination in seafood.
- The methodology can be extended for antibody characterization and detection of other marine toxins.
