Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Affinity and Avidity01:41

Affinity and Avidity

Overview
Affinity Chromatography01:03

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
Antibody Actions01:26

Antibody Actions

Antibodies, or immunoglobulins, are critical players in the immune system's arsenal against invading pathogens. Produced by B cells and plasma cells, their primary role is to detect and bind to specific antigens, molecules found on the surface of pathogens like bacteria or viruses. Beyond antigen recognition, antibodies perform several vital functions that contribute to immune defense.
Neutralization
Antibodies can bind to pathogens, preventing them from infecting host cells. This process...
Cross-reactivity00:42

Cross-reactivity

Overview
Hybridoma Technology01:31

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reducing antimicrobial resistance burden from livestock production by targeting pig gut health.

Frontiers in veterinary science·2026
Same author

Translational human gut microbiome research: What are the missing pieces of the puzzle?

Journal of translational medicine·2026
Same author

From "synthetic" to defined microbial communities for clearer terminology.

Nature communications·2026
Same author

Strong mucosal adhesion enhances the anti-infective effectiveness of T4-like phages.

Applied and environmental microbiology·2026
Same author

The ecological gain of using bioactivated carbon and ozone sewage treatments assessed using freshwater microcosms.

Water research·2026
Same author

High stability of the genome of <i>Akkermansia muciniphila</i> Muc<sup>T</sup> under long-term culturing conditions.

Microbiology spectrum·2026

Related Experiment Video

Updated: Jun 2, 2026

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study
07:53

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study

Published on: August 16, 2019

Alternative affinity tools: more attractive than antibodies?

Vincent J B Ruigrok1, Mark Levisson, Michel H M Eppink

  • 1Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands. Vincent.Ruigrok@wur.nl

The Biochemical Journal
|April 29, 2011
PubMed
Summary

Innovative affinity tools like aptamers and engineered binding proteins offer advantages over traditional antibodies, including lower costs and improved stability. These alternatives are rapidly advancing for diverse applications in research and medicine.

More Related Videos

Antibody Binding Specificity for Kappa (V&#954;) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study
11:10

Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study

Published on: June 29, 2016

Related Experiment Videos

Last Updated: Jun 2, 2026

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study
07:53

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study

Published on: August 16, 2019

Antibody Binding Specificity for Kappa (V&#954;) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study
11:10

Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study

Published on: June 29, 2016

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Biochemistry

Background:

  • Antibodies are widely used affinity tools but face limitations in cost and stability.
  • Alternative affinity tools, including aptamers, engineered binding proteins, and molecular imprinted polymers, are gaining traction.
  • These alternatives offer efficient production and in vitro selection methods.

Purpose of the Study:

  • To compare the properties of conventional antibodies with innovative affinity tools.
  • To review recent advances in the development and application of novel affinity tools.
  • To provide an outlook on future developments in the field of affinity tools.

Main Methods:

  • Literature review and comparative analysis of different affinity tool classes.
  • Discussion of in vitro selection and optimization procedures for aptamers and engineered proteins.
  • Examination of applications in medical and niche research areas.

Main Results:

  • Alternative affinity tools demonstrate advantages in production efficiency and stability.
  • High-throughput optimization enables enhanced chemical and physical properties of aptamers and engineered proteins.
  • Increasing applications in diagnostics, therapeutics, drug delivery, and specialized research areas are noted.

Conclusions:

  • Innovative affinity tools present viable alternatives to antibodies, addressing key limitations.
  • The development of nucleic acid- and protein-based affinity tools is rapidly progressing.
  • Future advancements are anticipated in both medical and niche applications for these novel tools.