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Updated: Jun 2, 2026

Enzymatic Cascade Reactions for the Synthesis of Chiral Amino Alcohols from L-lysine
Published on: February 16, 2018
D-Ornithine coopts pyrrolysine biosynthesis to make and insert pyrroline-carboxy-lysine
Susan E Cellitti1, Weijia Ou, Hsien-Po Chiu
1Genomics Institute of the Novartis Research Foundation, San Diego, California, USA.
Abstract:
D-ornithine has previously been suggested to enhance the expression of pyrrolysine-containing proteins. We unexpectedly discovered that uptake of D-ornithine results in the insertion of a new amino acid, pyrroline-carboxy-lysine (Pcl) instead of the anticipated pyrrolysine (Pyl). Our feeding and biochemical studies point to specific roles of the poorly understood Pyl biosynthetic enzymes PylC and PylD in converting L-lysine and D-ornithine to Pcl and confirm intermediates in the biosynthesis of Pyl.
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