Related Experiment Video
Updated: Jun 2, 2026
09:53
Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage
Published on: February 7, 2021
In situ monitoring of single molecule binding reactions with time-lapse atomic force microscopy on functionalized DNA
Na Wu1, Xingfei Zhou, Daniel M Czajkowsky
1Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, 201800, China.
Nanoscale
|April 29, 2011
Abstract:
Individual biomolecular binding events were recorded in situ by combining time-lapse atomic force microscopy and DNA origami. Single streptavidin molecules bound to specifically biotinyated DNA origami were simply counted as a function of time to obtain a direct measure of the binding rate.
