Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Read speech voice quality and disfluency in individuals with recent suicidal ideation or suicide attempt.

Speech communication·2026
Same author

Systemic inflammatory perturbations triggered by neuropathic pain in L5 compressed mouse and rat model.

Journal of orthopaedic translation·2026
Same author

Artificial anterolateral ligament reconstruction provides similar knee kinematics as compared to an autologous reconstruction and a lateral extra-articular tenodesis.

Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA·2025
Same author

Outperformance of Combined Artificial Anterolateral Ligament and ACL Reconstruction Compared With Isolated Artificial ACL Reconstruction in Knees With Anterolateral Structure and ACL Deficiency: A Biomechanical Analysis.

Orthopaedic journal of sports medicine·2025
Same author

Clinically significant changes in anal sphincter hiatal area in patients with gestational diabetes mellitus and pelvic organ prolapse.

World journal of diabetes·2024
Same author

Exploratory analysis of high-dose corticosteroid therapy on epileptic encephalopathy with spike-and-wave activation in sleep.

Frontiers in pediatrics·2024
Same journal

Running exercise alleviates chronic heart failure by promoting cardiomyocyte autophagic flux through the NEAT1-QKI affecting Beclin1/LC3B mRNA stability.

Biology direct·2026
Same journal

The PTHR1/PKA/CREB1 axis promotes osteosarcoma progression by activating the PVT1/miR-590-3p/AXIN2 ceRNA network to induce epithelial-mesenchymal transition.

Biology direct·2026
Same journal

Identification and prognostic analysis of genes related to CTNNB1 mutations in hepatocellular carcinoma.

Biology direct·2026
Same journal

TrxR1 inhibition sensitizes hepatocellular carcinoma to Motesanib via an autophagy-ROS-JNK/ER stress axis.

Biology direct·2026
Same journal

Integrated microbiome-metabolome analysis implicates Acinetobacter guillouiae in arachidonic acid metabolic remodeling and endometrial cancer cell proliferation.

Biology direct·2026
Same journal

Comprehensive multi-omics analysis reveals a fatty acid metabolism gene signature for prognostic assessment and immunotherapy in nasopharyngeal carcinoma, and identifies ABCC1 as a potential novel therapeutic target.

Biology direct·2026
See all related articles

Related Experiment Video

Updated: Jun 2, 2026

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
08:35

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Published on: July 17, 2021

Statistical methods for detecting periodic fragments in DNA sequence data.

Julien Epps1, Hua Ying, Gavin A Huttley

  • 1School of Electrical Engineering and Telecommunications, The University of New South Wales, Sydney, NSW 2052, Australia. j.epps@unsw.edu.au

Biology Direct
|April 30, 2011
PubMed
Summary
This summary is machine-generated.

Accurate identification of period-10 dinucleotides is crucial for understanding genome organization. New methods reveal significant portions of yeast and mouse genomes contain these nucleosome positioning sequences (NPS).

More Related Videos

Rare Event Detection Using Error-corrected DNA and RNA Sequencing
10:36

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

DNA Fingerprinting of Mycobacterium leprae Strains Using Variable Number Tandem Repeat (VNTR) - Fragment Length Analysis (FLA)
09:39

DNA Fingerprinting of Mycobacterium leprae Strains Using Variable Number Tandem Repeat (VNTR) - Fragment Length Analysis (FLA)

Published on: July 15, 2011

Related Experiment Videos

Last Updated: Jun 2, 2026

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
08:35

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Published on: July 17, 2021

Rare Event Detection Using Error-corrected DNA and RNA Sequencing
10:36

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

DNA Fingerprinting of Mycobacterium leprae Strains Using Variable Number Tandem Repeat (VNTR) - Fragment Length Analysis (FLA)
09:39

DNA Fingerprinting of Mycobacterium leprae Strains Using Variable Number Tandem Repeat (VNTR) - Fragment Length Analysis (FLA)

Published on: July 15, 2011

Area of Science:

  • Genomics
  • Bioinformatics
  • Molecular Biology

Background:

  • Period 10 dinucleotides influence DNA's ability to form nucleosomes.
  • Accurate identification of periodic signals in DNA is essential for understanding genome organization.
  • Existing methods for identifying periodic signals lack detailed consideration of requirements and confirmatory testing.

Purpose of the Study:

  • To compare the accuracy and suitability of different methods for identifying period-10 dinucleotides.
  • To develop and evaluate confirmatory testing procedures for period detection.
  • To assess the prevalence of period-10 nucleosome positioning sequences (NPS) in whole genomes.

Main Methods:

  • Compared autocorrelation, discrete Fourier transform (DFT), integer period discrete Fourier transform (IPDFT), and a Hybrid measure for period estimation.
  • Evaluated statistical significance procedures, with blockwise bootstrap proving superior.
  • Applied methods to synthetic data and ChIP-chip data from yeast, followed by whole-genome analysis of yeast and mouse.

Main Results:

  • The Hybrid technique showed superior exploratory period estimation for eroded or approximate period-10 signals.
  • Blockwise bootstrap with IPDFT demonstrated the greatest statistical power for confirmatory testing.
  • Approximately 21% of yeast and 19% of mouse genomes were identified as being spanned by period-10 NPS.

Conclusions:

  • The Hybrid method is effective for estimating dominant periods, especially with eroded periodicity.
  • Blockwise bootstrap is a powerful significance measure for period detection.
  • A substantial proportion of yeast and mouse genomes contain period-10 NPS, with potential biological significance yet to be determined.