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Related Experiment Video

Updated: Jun 2, 2026

Live Imaging of Early Cardiac Progenitors in the Mouse Embryo
07:02

Live Imaging of Early Cardiac Progenitors in the Mouse Embryo

Published on: July 12, 2022

Long-term imaging of mouse embryos using adaptive harmonic generation microscopy.

Anisha Thayil1, Tomoko Watanabe, Alexander Jesacher

  • 1Department of Engineering Science, University of Oxford, Parks Road, Oxford, Oxfordshire OX1 3PJ, United Kingdom.

Journal of Biomedical Optics
|May 3, 2011
PubMed
Summary

We developed an adaptive harmonic generation microscope for long-term, 3D imaging of mouse embryos. This technique allows observation of normal embryo development and dynamic events like blastocyst formation.

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Area of Science:

  • Developmental Biology
  • Microscopy Techniques
  • Biophotonics

Background:

  • Long-term imaging of early mouse embryos is crucial for understanding developmental processes.
  • Existing microscopy techniques may be limited in resolution or invasiveness for prolonged embryo observation.

Purpose of the Study:

  • To present an adaptive harmonic generation (HG) microscope system for detailed, long-term, 3D imaging of mouse embryos.
  • To characterize HG signals from different developmental stages of mouse embryos.
  • To assess the impact of imaging on normal embryo development.

Main Methods:

  • Development of an adaptive harmonic generation microscope.
  • Establishment of culture techniques for long-term mouse embryo observation.
  • Characterization of second and third harmonic generation signals.

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Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy
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Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy

Published on: November 21, 2016

Contrast Imaging in Mouse Embryos Using High-frequency Ultrasound
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Contrast Imaging in Mouse Embryos Using High-frequency Ultrasound

Published on: March 4, 2015

Related Experiment Videos

Last Updated: Jun 2, 2026

Live Imaging of Early Cardiac Progenitors in the Mouse Embryo
07:02

Live Imaging of Early Cardiac Progenitors in the Mouse Embryo

Published on: July 12, 2022

Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy
07:27

Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy

Published on: November 21, 2016

Contrast Imaging in Mouse Embryos Using High-frequency Ultrasound
10:39

Contrast Imaging in Mouse Embryos Using High-frequency Ultrasound

Published on: March 4, 2015

  • Imaging of mouse embryos from 0.5 to 5.5 days post-fertilization.

    • Main Results:

    • Detailed characterization of harmonic generation signals from mouse embryos.
    • Second harmonic generation images revealed central spindles during cytokinesis.
    • Third harmonic generation images visualized cellular structures like lipid droplets, nucleoli, and plasma membranes.
    • Mouse embryos developed normally during one-day-long discontinuous HG imaging.

    Conclusions:

    • Adaptive harmonic generation microscopy enables non-invasive, long-term, 3D imaging of mouse embryos.
    • The technique allows observation of dynamic developmental events, including morula compaction and blastocyst formation.
    • This method supports normal embryonic development during imaging, facilitating detailed study of early embryogenesis.