Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Allosteric modulation of the catalytic VYD loop in Slingshot by its N-terminal domain underlies both Slingshot auto-inhibition and activation.

The Journal of biological chemistry·2018

Same author

Isolated unilateral pulmonary vein atresia with hemoptysis in a child: A case report and literature review.

Medicine·2018

Same author

Clinical factors associated with intestinal strangulating obstruction and recurrence in adhesive small bowel obstruction: A retrospective study of 288 cases.

Medicine·2018

Same author

[Determination of organic acids in 1, 2-butylene oxide products by valve switch-ion chromatography].

Se pu = Chinese journal of chromatography·2018

Same author

Disruption of Planar Cell Polarity Pathway Attributable to Valproic Acid-Induced Congenital Heart Disease through Hdac3 Participation in Mice.

Chinese medical journal·2018

Same author

Multispectral and large bandwidth achromatic imaging with a single diffractive photon sieve.

Optics express·2018

Search research articles

Related Experiment Video

Updated: Jun 2, 2026

How to Build a Laser Speckle Contrast Imaging (LSCI) System to Monitor Blood Flow

How to Build a Laser Speckle Contrast Imaging (LSCI) System to Monitor Blood Flow

Published on: November 11, 2010

Choroidal laser Doppler flowmeter with enhanced sensitivity based on a scattering plate.

Chuan Wang¹, Zhihua Ding, Martial Geiser

¹Zhejiang University, State Key Lab of Modern Optical Instrumentation, Yuquan Campus, 38 Zheda Road, Hangzhou 310027, China.

Journal of Biomedical Optics

|May 3, 2011

Summary

A new portable laser Doppler flowmeter (LDF) uses a scattering plate to enhance sensitivity for measuring choroidal blood flow. This portable device offers improved detection of subtle blood flow changes in the eye.

More Related Videos

Scanning Light Scattering Profiler (SLPS) Based Methodology to Quantitatively Evaluate Forward and Backward Light Scattering from Intraocular Lenses

Scanning Light Scattering Profiler (SLPS) Based Methodology to Quantitatively Evaluate Forward and Backward Light Scattering from Intraocular Lenses

Published on: June 6, 2017

Related Experiment Videos

Last Updated: Jun 2, 2026

How to Build a Laser Speckle Contrast Imaging (LSCI) System to Monitor Blood Flow

How to Build a Laser Speckle Contrast Imaging (LSCI) System to Monitor Blood Flow

Published on: November 11, 2010

Scanning Light Scattering Profiler (SLPS) Based Methodology to Quantitatively Evaluate Forward and Backward Light Scattering from Intraocular Lenses

Scanning Light Scattering Profiler (SLPS) Based Methodology to Quantitatively Evaluate Forward and Backward Light Scattering from Intraocular Lenses

Published on: June 6, 2017

Area of Science:

Ophthalmology
Biomedical Engineering
Medical Optics

Background:

Choroidal blood flow monitoring is crucial for diagnosing and managing various eye conditions.
Existing laser Doppler flowmetry (LDF) systems face limitations in sensitivity and penetration depth.
Developing portable and enhanced-sensitivity devices is essential for accessible ophthalmic diagnostics.

Purpose of the Study:

To develop and evaluate a portable choroidal laser Doppler flowmeter (LDF) with enhanced sensitivity.
To investigate the use of a scattering plate to improve photon detection and blood flow measurement.
To assess the trade-offs between sensitivity enhancement and spatial resolution.

Main Methods:

Development of a portable LDF system operating at a 780 nm center wavelength.
Integration of a scattering plate positioned in front of the eye to enhance Doppler scattering.
Theoretical analysis of the detection mechanism and sensitivity enhancement.
In vivo performance evaluation using measurements on ten healthy volunteers.

Main Results:

The portable LDF achieved enhanced sensitivity for measuring choroidal blood flow.
The scattering plate increased the collection of backscattering light at high Doppler shift frequencies.
A trade-off was observed, with decreased spatial resolution and total signal measured.
The system demonstrated potential for detecting very slight alterations in blood flow.

Conclusions:

The developed portable LDF with a scattering plate offers enhanced sensitivity for choroidal blood flow measurement.
The system is particularly useful for applications prioritizing the detection of subtle flow changes over high spatial resolution.
This technology may aid in the early detection and monitoring of ophthalmic diseases characterized by altered choroidal vasculature.