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Assays for the Identification of Novel Antivirals against Bluetongue Virus
12:02

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Published on: October 11, 2013

Novel bluetongue virus serotype from Kuwait.

Sushila Maan1, Narender S Maan, Kyriaki Nomikou

  • 1Institute for Animal Health, Woking, UK.

Emerging Infectious Diseases
|May 3, 2011
PubMed
Summary

Bluetongue virus (BTV) was detected in sheep and goats in Kuwait in 2010. A novel BTV serotype, KUW2010/02, was identified from a sheep exhibiting mild symptoms.

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Area of Science:

  • Veterinary Virology
  • Molecular Epidemiology
  • Arbovirology

Background:

  • Bluetongue virus (BTV) is a re-emerging pathogen affecting ruminants globally.
  • Kuwait is a region with potential for arbovirus transmission due to its climate and livestock trade.

Purpose of the Study:

  • To investigate the presence of bluetongue virus in sheep and goats in Kuwait.
  • To characterize a BTV isolate from Kuwait.

Main Methods:

  • Serological testing of sheep and goats for BTV antibodies.
  • Real-time reverse transcription-PCR (rRT-PCR) for BTV RNA detection.
  • Nucleotide sequencing of the BTV isolate.

Main Results:

  • Serological evidence of BTV infection was found in sheep and goats.
  • One sheep tested positive for BTV by rRT-PCR, yielding isolate KUW2010/02.
  • Sequence analysis confirmed KUW2010/02 as a novel BTV serotype.

Conclusions:

  • This study confirms BTV circulation in Kuwaiti livestock.
  • The identification of a novel BTV serotype highlights the need for ongoing surveillance and diagnostic updates.