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Published on: December 9, 2022
Application of trichloroacetic acid-acetone precipitation method for protein extraction in bone tissue
Cui Yong-feng1, Yang Zhen, Zhu Bao-hua
1Department of Orthopedics, the First People's Hospital of Xiaoshan District, Hangzhou 311200, China. guke120@hotmail.com
Objective:
To explore the feasibility and efficiency of extracting protein from bone tissue using trichloroacetic acid (TCA)-acetone precipitation method.
Methods:
Hydrochloric acid (HCL) decalcification method and TCA-acetone precipitation method were separately used for bone protein extraction. The efficiencies of these two methods were compared.
Results:
TCA-acetone precipitation method had significantly higher extraction efficiency. Compared with HCL decalcification method, it had less pollution from bone marrow hematopoietic tissue. Protein band distribution was similar between these two methods.
Conclusion:
TCA-acetone precipitation method is useful for bone proteomics research.
