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Updated: Jun 2, 2026

Patch-Clamp Techniques for Single Endolysosomal Vesicle Analysis
Published on: April 4, 2025
The endo-lysosomal system as an NAADP-sensitive acidic Ca(2+) store: role for the two-pore channels
Sandip Patel1, Latha Ramakrishnan, Taufiq Rahman
1Department of Cell and Developmental Biology, University College London, London, UK. patel.s@ucl.ac.uk
Abstract:
Accumulating evidence suggests that the endo-lysosomal system provides a substantial store of Ca(2+) that is tapped by the Ca(2+)-mobilizing messenger, NAADP. In this article, we review evidence that NAADP-mediated Ca(2+) release from this acidic Ca(2+) store proceeds through activation of the newly described two-pore channels (TPCs). We discuss recent advances in defining the sub-cellular targeting, topology and biophysics of TPCs. We also discuss physiological roles and the evolution of this ubiquitous ion channel family.
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