A novel amperometric hydrogen peroxide biosensor based on electrospun Hb-collagen composite

Area of Science:

• Biomedical Engineering
• Electrochemistry
• Materials Science

Background:

• Hemoglobin (Hb) is crucial for oxygen transport.
• Efficient electron transfer is key for biosensor performance.
• Collagen provides a biocompatible matrix for biomolecule immobilization.

Purpose of the Study:

• To fabricate a three-dimensional hemoglobin-collagen microbelt modified electrode.
• To enhance the direct electron transfer of immobilized hemoglobin.
• To develop a sensitive and stable biosensor for hydrogen peroxide detection.

Main Methods:

• Electrospinning technique for fabricating 3D collagen microbelts.
• Immobilization of hemoglobin onto the collagen microbelts.
• Electrochemical characterization and amperometric detection of H₂O₂.

Main Results:

• Facilitated direct electron transfer of immobilized Hb with a rate constant (k(s)) of 270.6 s⁻¹.
• Excellent bioelectrocatalytic activity towards H₂O₂ reduction.
• Linear amperometric response for H₂O₂ from 5 × 10⁻⁶ to 30 × 10⁻⁶ molL⁻¹, with a detection limit of 0.37 × 10⁻⁶ molL⁻¹.
• Apparent Michaelis-Menten constant (K(m)(app)) of 77.7 μmolL⁻¹.

Conclusions:

• The electrospun Hb-collagen microbelt electrode exhibits superior bioelectrocatalytic performance.
• The developed biosensor offers high sensitivity, good reproducibility, and stability for H₂O₂ sensing.
• This approach provides a promising platform for electrochemical biosensor development.