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Published on: December 9, 2022
Activity of a dry mist-generated hydrogen peroxide disinfection system against methicillin-resistant Staphylococcus
Nihal Piskin1, Guven Celebi, Canan Kulah
1Department of Infectious Diseases and Clinical Microbiology, Zonguldak Karaelmas University, Turkey. nihal_piskin@yahoo.com
Background:
The aim of this study was to evaluate the activity of a dry mist-generated hydrogen peroxide (DMHP) system (Sterinis; Gloster Sante Europe, Labege cedex, France) against methicillin-resistant Staphylococcus aureus (MRSA) and Acinetobacter baumannii.
Methods:
McFarland 0.5 suspensions of 2 test bacteria, either pure or containing 5% sterile serum, were prepared and inoculated onto sterile stainless steel disks. Each disk in a Petri dish-with the Petri dish cover either closed or open-was placed in different locations in an intensive care unit room. Quantitative cultures were performed after the cycle.
Results:
No growth occurred on the disks in the absence of a barrier, except 1 disk containing serum. Existence of a barrier, as a drawer or a covered Petri dish, caused failure in the disinfection activity. The mean reduction in initial log(10) bacterial count was lower for both of the test bacteria in presence of a barrier: 4.44- to 4.70-log(10) colony-forming units (cfu) decrease was observed in absence of a barrier, whereas 1.49- to 3.79-log(10) cfu decrease was observed in presence of a barrier. When the culture results were compared according to organic load content, the mean (±standard deviation) reduction of initial contamination in pure and in serum containing MRSA suspensions was 4.25 ± 1.20- and 3.34 ± 1.89-log(10) cfu, respectively. The mean (±standard deviation) reduction in pure and in serum containing A baumannii suspensions was 4.34 ± 0.89- and 3.87 ± 1.26-log(10) cfu, respectively. The differences were statistically significant (P < .001).
Conclusion:
Sterinis was capable of killing MRSA and A baumannii on open surfaces; however, it was not effective in closed or semiclosed areas. Presence of serum also caused failure in the disinfection activity of the system.
Insights
Dry mist hydrogen peroxide effectively kills MRSA and Acinetobacter baumannii on open surfaces but fails in enclosed areas or when organic matter is present. This highlights limitations in disinfection efficacy for certain conditions.
Area of Science:
- Microbiology
- Infectious Diseases
- Environmental Hygiene
Background:
- Methicillin-resistant Staphylococcus aureus (MRSA) and Acinetobacter baumannii are critical pathogens in healthcare settings.
- Effective disinfection strategies are crucial for preventing hospital-acquired infections.
Purpose of the Study:
- To evaluate the efficacy of a dry mist-generated hydrogen peroxide (DMHP) system against MRSA and A. baumannii.
- To determine the impact of barriers and organic load on DMHP disinfection effectiveness.
Main Methods:
- Bacterial suspensions of MRSA and A. baumannii were prepared with and without serum.
- Inoculated disks were placed in open or closed Petri dishes within an intensive care unit room.
- Quantitative cultures were performed post-treatment to assess bacterial reduction.
Main Results:
- DMHP demonstrated significant bacterial reduction on open surfaces (4.44-4.70 log10 cfu decrease).
- Disinfection efficacy was substantially reduced by barriers (1.49-3.79 log10 cfu decrease) and the presence of serum.
- Serum reduced bacterial log10 cfu reduction for MRSA and A. baumannii.
Conclusions:
- The DMHP system is effective against MRSA and A. baumannii on exposed surfaces.
- Disinfection is compromised in closed environments and in the presence of organic material.
- Limitations necessitate careful consideration of application scenarios for DMHP systems.
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