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Related Experiment Video

Updated: Jun 2, 2026

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

Published on: September 7, 2018

Variation in immunophenotype of lactating mice.

Jerry Wei¹, Christine Yee, Palaniappan Ramanathan

¹Faculty of Veterinary Science, The University of Sydney, Camperdown, NSW 2006, Australia. jerry.wei@sydney.edu.au

Journal of Reproductive Immunology

|May 3, 2011

Summary

This study explored immunological factors and genetic variations influencing mouse lactation performance. Certain plasma cytokines significantly predict milk production, highlighting their role in mammary gland function.

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Last Updated: Jun 2, 2026

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

Published on: September 7, 2018

Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland

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Published on: December 1, 2015

Operating and Biocontainment Procedures of a Facility for Laboratory Mice with a Natural Microbiome: Immunophenotyping Procedure

Operating and Biocontainment Procedures of a Facility for Laboratory Mice with a Natural Microbiome: Immunophenotyping Procedure

Published on: December 13, 2024

Area of Science:

Immunology
Lactation Biology
Genetics

Background:

Immunological factors are key in mammary gland remodeling during lactation and involution.
The interplay between immunology, genetics, and normal milk production remains poorly understood.

Purpose of the Study:

To investigate the relationship between immunological phenotypes and lactation performance in 11 inbred mouse strains.
To identify genetic components influencing lactation ability and potential cytokine biomarkers.

Main Methods:

Assessed lactation and immunological phenotypes across 11 inbred mouse strains.
Analyzed leukocyte subsets (e.g., CD8+ T lymphocytes, monocytes) and plasma cytokine profiles.
Utilized linear regression to model cytokine prediction of lactation performance.

Main Results:

No direct correlation found between splenic leukocyte fractions and lactation performance.
Significant strain-specific differences observed in CD8+ T lymphocytes and monocytes.
A subset of plasma cytokines (CCL2, CCL3, CCL5, CSF2, CSF3, IL10, IL15, IL1B, IL4, IL5, IL7, TNF) predicted lactation performance (R-sq=62%).
Strong correlations identified between splenic immune cells and plasma cytokine levels.

Conclusions:

Genetic variability significantly impacts immunological phenotypes in lactating mice.
Plasma cytokines are crucial predictors of lactation performance and may serve as biomarkers.
Findings provide a foundation for understanding cytokine roles in milk production.