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Related Concept Videos

Protein Transport to the Inner Chloroplast Membrane01:18

Protein Transport to the Inner Chloroplast Membrane

Proteins targeted to the inner chloroplast membrane, or plastid proteins, are transported by two general pathways: the stop-transfer and the re-insertion or post-import pathways. Most plastid proteins carry N-terminal transit sequences and internal import sequences targeting it to the specific chloroplast subcompartment. Proteins targeted by the stop-transfer pathway have internal hydrophobic sequences that inhibit their translocation into the stroma. As a result, these precursors are arrested...
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Cell Signaling in Plants

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Plasmodesmata02:32

Plasmodesmata

The organs in a multicellular organism’s body are made up of tissues formed by cells. To work together cohesively, cells must communicate. One way that cells communicate is through direct contact with other cells. The points of contact that connect adjacent cells are called intercellular junctions.Intercellular junctions are a feature of fungal, plant, and animal cells alike. However, different types of junctions are found in different kinds of cells. Intercellular junctions found in animal...

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Related Experiment Video

Updated: Jun 2, 2026

Confocal Microscopy Analysis of Protein Sorting to Plasmodesmata in Nicotiana benthamiana
05:54

Confocal Microscopy Analysis of Protein Sorting to Plasmodesmata in Nicotiana benthamiana

Published on: November 1, 2024

Arabidopsis plasmodesmal proteome.

Lourdes Fernandez-Calvino1, Christine Faulkner, John Walshaw

  • 1John Innes Centre, Norwich Research Park, Colney, Norwich, United Kingdom.

Plos One
|May 3, 2011
PubMed
Summary
This summary is machine-generated.

Plant cells communicate via plasmodesmata (PD), channels in cell walls. Proteomic analysis identified 1341 proteins in Arabidopsis PD, revealing membrane proteins and potential new signaling molecules for plant cell communication.

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Last Updated: Jun 2, 2026

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Published on: November 1, 2024

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Identification of Plasmodesmal Localization Sequences in Proteins In Planta
08:07

Identification of Plasmodesmal Localization Sequences in Proteins In Planta

Published on: August 15, 2017

Area of Science:

  • Plant Biology
  • Cell Biology
  • Proteomics

Background:

  • Plant cells require intercellular communication for coordinated function.
  • Plasmodesmata (PD) are crucial cell wall pores facilitating molecular transport between plant cells.

Purpose of the Study:

  • To perform a proteomic analysis of purified plasmodesmata from Arabidopsis suspension cells.
  • To identify proteins within plasmodesmata and validate the proteome for understanding cell-to-cell communication.

Main Methods:

  • Purification of plasmodesmata from Arabidopsis suspension cells.
  • Proteomic analysis using nano-liquid chromatography and Orbitrap mass spectrometry.
  • Validation of identified proteins using molecular and cell biology techniques.

Main Results:

  • Identification of 1341 proteins in the plasmodesmata proteome (PD-proteome).
  • The PD-proteome is enriched in membrane proteins and contains known and inferred PD proteins.
  • Discovery of three novel potential plasmodesmal proteins: receptor-like kinases, a tetraspanin, and an unknown protein.

Conclusions:

  • The plasmodesmata proteome is a valuable resource for studying plant cell communication.
  • Plasmodesmata contain a significant number of membrane proteins, including receptor-like proteins, suggesting roles in signaling.
  • The identified novel proteins offer new avenues for investigating plasmodesmata structure and function.