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Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

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Related Experiment Video

Updated: Jun 2, 2026

Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture (CARIC) Strategy
09:36

Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture (CARIC) Strategy

Published on: October 19, 2018

RNA captor: a tool for RNA characterization.

Christian Clepet1

  • 1URGV Plant Genomics, INRA UMR1165 UEVE/CNRS ERL 8196, Evry, France. clepet@evry.inra.fr

Plos One
|May 3, 2011
PubMed
Summary

A new RNA tagging method, RNA Captor, enhances the study of RNA molecules. This universal protocol improves the isolation of less-abundant RNAs, aiding genome annotation and transcript analysis.

Area of Science:

  • Molecular Biology
  • Genomics
  • RNA Biology

Background:

  • Characterizing RNA structure and function is challenging in the genome era.
  • Current genome annotation struggles with alternative transcripts and non-protein-coding genes.
  • Efficient tools are needed to isolate low-abundance or stable RNAs.

Purpose of the Study:

  • To develop a universal RNA tagging method for improved RNA characterization.
  • To establish protocols for RACE PCR and full-length cDNA library construction.
  • To enhance the sensitivity and applicability of RNA isolation techniques.

Main Methods:

  • Development of a universal RNA tagging method utilizing T4 RNA ligase 2 and specialized adapters.
  • Optimization of RNA tagging conditions and comparison with existing methods.

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PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins

Published on: July 2, 2010

Related Experiment Videos

Last Updated: Jun 2, 2026

Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture (CARIC) Strategy
09:36

Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture (CARIC) Strategy

Published on: October 19, 2018

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
11:52

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins
12:24

PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins

Published on: July 2, 2010

  • Application of the method for RACE PCR and full-length cDNA library construction.

    • Main Results:

    • A universal RNA tagging system, RNA Captor, was successfully developed.
    • Protocols for RACE PCR and full-length cDNA library construction were established.
    • Two large-scale full-length cDNA inventories were generated using this method.

    Conclusions:

    • RNA Captor offers a straightforward and accessible protocol for RNA analysis.
    • The method demonstrates higher sensitivity than previous techniques.
    • It is applicable to various RNA types, including mRNA, non-coding RNA, and cleavage sites, with potential for deep sequencing.