Related Experiment Video
Updated: Feb 9, 2026

Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy
Published on: November 11, 2025
Mapping signals that are important for nuclear and nucleolar localization in MCRS2
Chung-Wen Kuo1, Wen-Hung Wang, Shih-Tung Liu
1Department of Microbiology and Immunology, Chang-Gung University, Kwei-Shan, Taoyuan, 33302, Taiwan.
Abstract:
MCRS2 is an oncoprotein that is sequestered in the nucleolus. When in the nucleolus, it promotes the transcription of the rRNA gene. MCRS2 also brings proteins into the nucleolus to change their function. This study analyzes the sequence of MCRS2 and determines that the nuclear localization signal, which has the sequence KRKK, is situated between amino acids 66 and 69. Meanwhile, MCRS2 contains a bipartite nucleolar localization signal, which comprises a KKSK motif, located between amino acids 133 and 136, and a downstream 152-amino acid region, from amino acid 314 to 465. The results of this study are important to understand the function of MCRS2.
Related Concept Videos
Nuclear Localization Signals and Import
Nuclear Stability
To hold positively charged protons together...
Radioactivity and Nuclear Equations
A nuclide of an element has a specific number of protons and...
Nuclear Fusion
A helium nucleus has a mass that is 0.7% less than that of four hydrogen nuclei; this lost mass is converted into energy during the fusion. This reaction produces about...
Non-nuclear Inheritance
Nuclear Export of mRNA

