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Published on: November 10, 2015
Single strand conformation polymorphism is a sensitive method for screening nucleotide variations in Mycosphaerella
A Siah1, B Tisserant, L El Chartouni
1Univ. Ulle-Nord de France.
Communications in Agricultural and Applied Biological Sciences
|May 4, 2011
Summary
Single Strand Conformation Polymorphism (SSCP) analysis revealed high conservation of mating type sequences in Mycosphaerella graminicola. However, the study suggests differing polymorphism levels between the MAT1-1 and MAT1-2 mating type sequences.
Area of Science:
- Mycology
- Molecular Biology
- Plant Pathology
Background:
- Mycosphaerella graminicola causes Septoria tritici blotch, a significant wheat disease.
- Understanding mating type sequence polymorphism is crucial for fungal population genetics.
Purpose of the Study:
- To assess the molecular polymorphism of mating type sequences (mat1-1 and mat1-2) in Mycosphaerella graminicola.
- To evaluate the efficacy of Single Strand Conformation Polymorphism (SSCP) for detecting these variations.
Main Methods:
- Multiplex PCR amplification of partial mat1-1 and mat1-2 sequences.
- Restriction digestion with Taq1 followed by SSCP analysis.
- Sequencing of amplicons with divergent SSCP profiles.
Main Results:
- High conservation of mating type idiomorphs observed across 510 French strains and two reference isolates.
- SSCP successfully detected single nucleotide variations, indicating high sensitivity.
- MAT1-2 sequences exhibited a higher level of polymorphism compared to MAT1-1 sequences.
Conclusions:
- SSCP is a sensitive method for detecting single point mutations in M. graminicola mating type sequences.
- Mating type sequences are conserved at both sequence and population levels in this fungus.
- A notable difference in polymorphism levels exists between the two mating type sequences.
