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Atomic Force Microscopy

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

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Updated: Jun 2, 2026

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

Published on: May 24, 2017

Optimizing single DNA molecules manipulation by AFM.

F Long¹, C Wang, M Lü

¹School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai, China.

Journal of Microscopy

|May 4, 2011

Summary

This summary is machine-generated.

This study introduces an automated atomic force microscope method for single DNA manipulation, significantly improving efficiency for tasks like dissection and folding. The new technique offers the same performance as manual methods but is much faster.

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Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

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Published on: April 12, 2019

Area of Science:

Nanotechnology
Molecular Biology
Biophysics

Background:

Single DNA molecule manipulation using atomic force microscopy (AFM) offers molecular-level genetic analysis.
Current manual AFM DNA manipulation is labor-intensive and time-consuming, hindering broader applications.

Purpose of the Study:

To develop an optimized and automated method for single DNA manipulation using AFM.
To enhance the efficiency and accuracy of DNA nanomanipulation techniques.

Main Methods:

Implemented an image correlation technique for automatic thermal drift compensation.
Developed precise AFM tip movement control for various manipulation tasks (dissection, folding, picking).

Main Results:

Achieved an order of magnitude improvement in manipulation efficiency compared to manual methods.
Demonstrated comparable performance and accuracy to existing manual techniques.
Successfully executed complex DNA manipulations like dissection, folding, and picking.

Conclusions:

The developed automated AFM method significantly enhances the efficiency of single DNA manipulation.
This advancement enables high-efficiency nanomanipulation of complex biomolecules.
Facilitates deeper understanding of single biomolecule intrinsic properties.