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A radiochemical method for the determination of transketolase activity in erythrocyte hemolysates
Clinica Chimica Acta; International Journal of Clinical Chemistry
|December 15, 1978
Abstract:
A radiochemical method for the determination of transketolase activity is reported. It is based on incubation of erythrocyte hemolysates with radioactive ribose 5-phosphate followed by isolation of sedoheptulose 7-phosphate on anion-exchange columns. The experimental conditions are discussed and the presence of phosphatase activity in the incubation mixture is demonstrated. The proposed method is compared with a current colorimetric method. Reference values are given for the transketolase activity and the thiamine pyrophosphate effect.