Human dental pulp cell responses to new calcium silicate-based endodontic materials
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Updated: Jun 2, 2026
Accessing the Cytotoxicity and Cell Response to Biomaterials
Published on: July 8, 2021
1Department of Dentistry, Chung Shan Medical University Hospital, Taichung City, Taiwan.
Aim:
To evaluate human dental pulp cell responses to radiopaque dicalcium silicate cement and white-coloured mineral trioxide aggregate (WMTA).
Methodology:
Flow cytometry was employed to quantify the phase percentage of pulp cell cycle. Alamar Blue was used for real-time and repeated monitoring of cell proliferation. Reverse transcription-polymerase chain reaction was performed to determine gene expression in pulp cells cultured on the cements.
Results:
The cells cultured on the radiopaque dicalcium silicate cement had similar S and G2 phases in the cell cycle and proliferation to WMTA at all culture times. In addition, the two materials presented the same evolution with similar values in interleukin-1, inducible nitric oxide synthase, alkaline phosphatase, osteocalcin and bone sialoprotein gene expression at all culture times.
Conclusions:
The dental pulp cell responses to radiopaque dicalcium silicate cement were similar to those reported for WMTA in terms of cell cycle, proliferation, immunocompatibility and osteogenic differentiation.
