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Enlargement of the Plasma Membrane01:22

Enlargement of the Plasma Membrane

Cell division and enlargement are processes that require precise control. The control ensures that cell division cannot proceed unless the cell has grown to a specific size. A spherical, dividing cell requires an approximately 1.6X increase in its surface area to double its volume. The secretory pathway also has a significant role in cell membrane enlargement. Secretory vesicles that bud off from the Golgi apparatus and later fuse with the plasma membrane during exocytosis are a major source of...

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Labeling the plasma membrane with TMA-DPH.

Brad Chazotte

    Cold Spring Harbor Protocols
    |May 4, 2011
    PubMed
    Summary
    This summary is machine-generated.

    Trimethylamine-diphenylhexatriene (TMA-DPH) is a fluorescent probe for studying membrane dynamics. This protocol details its use in labeling the plasma membrane for advanced cellular analysis.

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    Area of Science:

    • Biophysics
    • Cell Biology
    • Membrane Dynamics

    Background:

    • Trimethylamine-diphenylhexatriene (TMA-DPH) is a fluorescent probe.
    • It classically labels the outer leaflet of membrane bilayers and plasma membranes.
    • TMA-DPH reports on membrane dynamics via fluorescence polarization and lifetime.

    Purpose of the Study:

    • To describe a protocol for using TMA-DPH.
    • To label the plasma membrane using TMA-DPH.
    • To enable studies on membrane dynamics and cellular processes.

    Main Methods:

    • Utilizing TMA-DPH as a fluorescent membrane probe.
    • Employing fluorescence polarization and fluorescence lifetime techniques.
    • Applying the probe to label the plasma membrane for cellular studies.

    Main Results:

    • TMA-DPH effectively labels the outer leaflet of the plasma membrane.
    • The probe facilitates the study of membrane dynamics.
    • It can be used to monitor exocytosis and endocytosis.

    Conclusions:

    • TMA-DPH is a versatile tool for plasma membrane labeling.
    • This protocol enables detailed investigation of membrane properties and functions.
    • The probe aids in understanding cellular membrane interactions and dynamics.