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Related Experiment Video

Updated: Jun 2, 2026

Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons
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Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons

Published on: October 8, 2014

Two-photon uncaging microscopy.

Masanori Matsuzaki, Haruo Kasai

    Cold Spring Harbor Protocols
    |May 4, 2011
    PubMed
    Summary

    Two-photon uncaging microscopy precisely releases neurotransmitters like glutamate to map receptor densities and study neuronal structures. This advanced technique enables detailed 3D analysis of brain slices and dendritic spines.

    Area of Science:

    • Neuroscience
    • Biophysics
    • Microscopy

    Background:

    • Two-photon excitation offers optical sectioning for localized neurotransmitter release.
    • This enables precise activation of receptors and intracellular signaling in 3D.
    • Applications include mapping receptor densities and studying neuronal structures like dendritic spines.

    Purpose of the Study:

    • To provide a protocol for two-photon uncaging microscopy.
    • To detail the development of a microscope for effective caged neurotransmitter release.
    • To demonstrate applications in hippocampal neurons.

    Main Methods:

    • Utilizing two-photon excitation for precise spatial and temporal control.
    • Developing specialized microscopy for uncaging caged neurotransmitters.

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    In Vivo Two-Color 2-Photon Imaging of Genetically-Tagged Reporter Cells in the Skin

    Published on: July 11, 2019

    Related Experiment Videos

    Last Updated: Jun 2, 2026

    Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons
    10:29

    Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons

    Published on: October 8, 2014

    In Vivo Two-photon Imaging Of Experience-dependent Molecular Changes In Cortical Neurons
    10:07

    In Vivo Two-photon Imaging Of Experience-dependent Molecular Changes In Cortical Neurons

    Published on: January 5, 2013

    In Vivo Two-Color 2-Photon Imaging of Genetically-Tagged Reporter Cells in the Skin
    05:45

    In Vivo Two-Color 2-Photon Imaging of Genetically-Tagged Reporter Cells in the Skin

    Published on: July 11, 2019

  • Combining uncaging with two-photon imaging for functional and structural analysis.

    • Main Results:

    • Demonstrated effective two-photon release of caged neurotransmitters.
    • Successfully mapped receptor densities in complex neural structures.
    • Visualized long-term structural and functional consequences of stimulated dendritic spines.

    Conclusions:

    • Two-photon uncaging microscopy is a powerful tool for neuroscience research.
    • It allows detailed investigation of neuronal function and structure in 3D.
    • The protocol facilitates advanced studies in cultured and isolated hippocampal neurons.