Isolation of human capillary endothelial cells from abdominal adipose tissue

This protocol outlines a technique to isolate human capillary endothelial cells from human abdominal wall adipose tissue. The relative lack of cell-type complexity within fat tissue makes it an attractive source of endothelial cells. In addition, collaboration with a local plastic surgeon can yield significant quantities of subcutaneous adipose tissue from liposuction without the need to be on call for cadaveric or post-surgical samples.