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Quantitative Analysis of Cancer Metastasis using an Avian Embryo Model
Published on: May 30, 2011
Diminished metastasis in tetraspanin CD151-knockout mice
Yoshito Takeda1, Qinglin Li, Alexander R Kazarov
1Dana-Farber Cancer Institute, Boston, MA 02215-5450, USA.
Blood
|May 4, 2011
Summary
Host CD151 deficiency significantly reduces lung metastasis by impairing tumor cell interactions with endothelial cells and their extracellular matrix. This highlights CD151
Area of Science:
- Oncology
- Cell Biology
- Immunology
Background:
- Tetraspanin CD151 on tumor cells promotes invasion and metastasis.
- The role of host CD151 in metastasis is not well understood.
Purpose of the Study:
- To investigate the role of host CD151 in experimental lung metastasis.
- To elucidate the mechanisms by which host CD151 influences tumor cell metastasis.
Main Methods:
- Utilized CD151-null mice and wild-type littermates.
- Assessed experimental lung metastasis of Lewis lung carcinoma and B16F10 melanoma cells.
- Analyzed tumor cell adhesion, transendothelial migration, and endothelial cell signaling in vitro and in vivo.
Main Results:
- CD151-null mice exhibited significantly reduced lung metastasis.
- Deficiency in host CD151 impaired tumor cell adhesion and migration to lung endothelial cells.
- Host CD151 affects endothelial cell extracellular matrix production and tumor-endothelial interactions.
Conclusions:
- Host CD151 plays a critical role in supporting lung metastasis.
- Impaired tumor-endothelial interactions, particularly involving extracellular matrix, contribute to reduced metastasis in CD151-null hosts.
- Host CD151's role in metastasis is independent of local tumor growth and immune surveillance.
