Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Exon Recombination02:32

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Transgenic Plants02:50

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Antibiotic Selection00:57

Antibiotic Selection

Overview
Mutation, Gene Flow, and Genetic Drift01:09

Mutation, Gene Flow, and Genetic Drift

In a population that is not at Hardy-Weinberg equilibrium, the frequency of alleles changes over time. Therefore, any deviations from the five conditions of Hardy-Weinberg equilibrium can alter the genetic variation of a given population. Conditions that change the genetic variability of a population include mutations, natural selection, non-random mating, gene flow, and genetic drift (small population size).Mechanisms of Genetic VariationThe original sources of genetic variation are mutations,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

How to successfully express insect microsomal P450 using a baculovirus system.

Insect biochemistry and molecular biology·2026
Same author

A chromosome-level genome assembly and multi-omics analysis reveal fenpropathrin resistance mechanisms in the turnip aphid, Lipaphis erysimi.

Pest management science·2026
Same author

Challenges facing the management of pesticide resistance in weeds, diseases and insect pests in European agriculture and the future of effective IPM implementation.

Pest management science·2026
Same author

The cytochrome P450s CYP6CY3 and CYP6CY4 confer resistance to flupyradifurone in the green peach aphid Myzus persicae.

Insect biochemistry and molecular biology·2026
Same author

Interspecific comparison reveals disparities in insecticide adaptive evolutionary capacity in insect pests.

Pest management science·2026
Same author

Expanded UDP-glycosyltransferase gene clusters underlie insecticide detoxification in Spodoptera frugiperda.

Insect biochemistry and molecular biology·2025

Related Experiment Video

Updated: Jun 2, 2026

Using the GAL4-UAS System for Functional Genetics in Anopheles gambiae
06:29

Using the GAL4-UAS System for Functional Genetics in Anopheles gambiae

Published on: April 15, 2021

Gene amplification and insecticide resistance.

Chris Bass1, Linda M Field

  • 1Department of Biological Chemistry, Rothamsted Research, Harpenden, Herts, UK. chris.bass@bbsrc.ac.uk

Pest Management Science
|May 4, 2011
PubMed
Summary

Gene duplication and amplification drive pesticide resistance in arthropods through enhanced metabolism and target-site mutations. This review covers key developments in insecticide and acaricide resistance mechanisms.

Area of Science:

  • Entomology
  • Molecular Biology
  • Genetics

Background:

  • Arthropod pesticide resistance evolves via enhanced detoxification or target-site insensitivity.
  • Gene duplication/amplification is a key mechanism for enhanced metabolic enzyme production.
  • This genetic mechanism is implicated in resistance across major enzyme families like P450s, esterases, and GSTs.

Purpose of the Study:

  • To review the role of gene duplication and amplification in arthropod pesticide resistance.
  • To highlight recent advancements in understanding P450-mediated and target-site resistance.
  • To provide an overview of insecticide and acaricide resistance in insect and mite pests.

Main Methods:

  • Literature review of studies on gene duplication/amplification in pesticide resistance.

More Related Videos

Delivery of Nucleic Acids through Embryo Microinjection in the Worldwide Agricultural Pest Insect, Ceratitis capitata
09:45

Delivery of Nucleic Acids through Embryo Microinjection in the Worldwide Agricultural Pest Insect, Ceratitis capitata

Published on: October 1, 2016

Topical Application Bioassay to Quantify Insecticide Toxicity for Mosquitoes and Fruit Flies
09:37

Topical Application Bioassay to Quantify Insecticide Toxicity for Mosquitoes and Fruit Flies

Published on: January 19, 2022

Related Experiment Videos

Last Updated: Jun 2, 2026

Using the GAL4-UAS System for Functional Genetics in Anopheles gambiae
06:29

Using the GAL4-UAS System for Functional Genetics in Anopheles gambiae

Published on: April 15, 2021

Delivery of Nucleic Acids through Embryo Microinjection in the Worldwide Agricultural Pest Insect, Ceratitis capitata
09:45

Delivery of Nucleic Acids through Embryo Microinjection in the Worldwide Agricultural Pest Insect, Ceratitis capitata

Published on: October 1, 2016

Topical Application Bioassay to Quantify Insecticide Toxicity for Mosquitoes and Fruit Flies
09:37

Topical Application Bioassay to Quantify Insecticide Toxicity for Mosquitoes and Fruit Flies

Published on: January 19, 2022

  • Focus on mechanisms involving metabolic enzymes and target-site mutations.
  • Analysis of resistance in insect and mite pest populations.

    • Main Results:

    • Gene duplication/amplification significantly contributes to enhanced detoxification of pesticides.
    • This mechanism also plays a role in target-site resistance, reducing pesticide sensitivity.
    • Recent research shows its involvement in cytochrome P450-mediated resistance.

    Conclusions:

    • Gene duplication and amplification are critical evolutionary routes to pesticide resistance in arthropods.
    • Understanding these genetic mechanisms is crucial for managing insecticide and acaricide resistance.
    • Further research is needed to explore the full extent of gene duplication/amplification in pest resistance.