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Related Experiment Video

Updated: Jun 2, 2026

Automated Counterflow Centrifugal System for Small-Scale Cell Processing
04:49

Automated Counterflow Centrifugal System for Small-Scale Cell Processing

Published on: December 12, 2019

Development and implementation of a perfusion-based high cell density cell banking process.

Yiwen Tao1, Jennifer Shih, Marty Sinacore

  • 1Cell Culture Development, Biogen Idec Inc, San Diego, CA 92122, USA.

Biotechnology Progress
|May 4, 2011
PubMed
Summary
This summary is machine-generated.

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A novel high cell density (HD) perfusion cell banking process streamlines upstream operations, saving up to 9 days. This method enables direct inoculation into bioreactors, simplifying scale-up and manufacturing.

Area of Science:

  • Biotechnology
  • Cell Culture Technology
  • Bioprocess Engineering

Background:

  • Traditional cell inoculum expansion involves multiple shake flask steps, increasing process time and complexity.
  • Optimizing upstream unit operations is crucial for efficient biopharmaceutical manufacturing.

Purpose of the Study:

  • To develop and validate a high cell density (HD) perfusion cell banking process.
  • To reduce time and simplify upstream operations in cell culture scale-up.

Main Methods:

  • Developed and optimized HD perfusion cultures in a disposable Wave bioreactor system.
  • Optimized perfusion rate, rocking speed, and aeration to achieve high cell densities and viability.
  • Froze cells at HD (90-100 × 10^6 viable cells/mL) in CryoTube vials.

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Published on: July 20, 2022

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Last Updated: Jun 2, 2026

Automated Counterflow Centrifugal System for Small-Scale Cell Processing
04:49

Automated Counterflow Centrifugal System for Small-Scale Cell Processing

Published on: December 12, 2019

Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture
10:00

Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture

Published on: July 20, 2022

Main Results:

  • Achieved peak cell densities exceeding 20 × 10^6 cells/mL with ≥ 90% cell viability.
  • Eliminated the need for intermediate shake flask operations, saving up to 9 days in inoculum expansion.
  • Successfully implemented the HD cell banking process in a 2000 L scale manufacturing facility.

Conclusions:

  • The developed HD perfusion cell banking process offers significant time savings and operational simplification.
  • High-quality HD cell banks enable direct inoculation into bioreactors, streamlining the inoculum expansion train.
  • The disposable perfusion system and robust cell banks facilitate successful large-scale manufacturing implementation.