Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 2, 2026

Modified Mouse Embryonic Stem Cell based Assay for Quantifying Cardiogenic Induction Efficiency

Modified Mouse Embryonic Stem Cell based Assay for Quantifying Cardiogenic Induction Efficiency

Published on: April 22, 2011

Modified mouse embryonic stem cell based assay for quantifying cardiogenic induction efficiency.

Ada Ao¹, Charles H Williams, Jijun Hao

¹Division of Cardiovascular Medicine, Department of Medicine, Vanderbilt University School of Medicine.

Journal of Visualized Experiments : Jove

|May 5, 2011

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Zebrafish swimming towards cures: a scalable NAM platform for drug discovery.

Drug discovery today·2026

Same author

Optimization of Small-Molecule Wnt Pathway Activation with CHIR99021 for Human Induced Pluripotent Stem Cell Differentiation to Cardiomyocytes.

The National high school journal of science·2026

Same author

Inhibition of GPR68 induces ferroptosis in diffuse intrinsic pontine gliomas.

Frontiers in oncology·2026

Same author

Beyond Membrane Remodeling: Organelle Crosstalk and Convergent Pathology in Centronuclear Myopathy.

Muscles (Basel, Switzerland)·2026

Same author

Mesenchymal Stem Cell-Derived Exosomal miRNAs in Skin Repair and Rejuvenation.

Genes·2026

Same author

Detection of a Single Measles Infection Using Untargeted Ultra-Deep Metagenomic Sequencing of Wastewater in Cook County, Illinois.

NEJM evidence·2026

Same journal

A Video Protocol of a Randomized Controlled Clinical Trial - Electrochemotherapy of Cutaneous Metastases with Reduced Dose Bleomycin (BLESS Trial).

Journal of visualized experiments : JoVE·2026

Same journal

A Standardized Ex Vivo Porcine Oromucosal Model for Evaluating Peptide Fluxes.

Journal of visualized experiments : JoVE·2026

Same journal

Lightweight English Text Classification with Deep Learning Based on Complex System Theory.

Journal of visualized experiments : JoVE·2026

Same journal

Integrating Artificial Intelligence-Assisted Translation Support into English Courses: Effects on Translation Accuracy, Perceived Stress, and Anxiety.

Journal of visualized experiments : JoVE·2026

Same journal

A Toxin-Based Counter-Selection System for Markerless Gene Deletion and High-Density Tn5 Transposon Mutagenesis in Pectobacterium brasiliense.

Journal of visualized experiments : JoVE·2026

Same journal

Seamless Multimodal Human-Robot Communication: Integration Techniques in Human-Computer Interaction.

Journal of visualized experiments : JoVE·2026

See all related articles

This study presents a mouse embryonic stem cell assay to pinpoint critical Wnt/β-catenin and BMP signaling timings for efficient cardiac differentiation. The 96-well format enables rapid quantification and potential for high-throughput analysis of stem cell differentiation.

Area of Science:

Stem cell biology
Developmental biology
Signaling pathways

Background:

Pluripotent stem cell differentiation is regulated by complex signaling pathways.
Assessing the impact of signaling events on differentiation efficiency presents a methodological challenge.

Purpose of the Study:

To develop a robust assay for identifying critical time windows of signaling pathway activation during cardiogenic induction.
To quantify differentiation efficiency using a high-throughput compatible method.

Main Methods:

Utilized a mouse embryonic stem (ES) cell based assay.
Employed a 96-well plate format for scoring contracting embryonic bodies (EBs).
Investigated Wnt/β-catenin and Bone Morphogenetic Protein (BMP) signal activation timing during cardiogenesis.

More Related Videos

An Efficient Transgenesis Approach for Gene Delivery in the Mouse Embryonic Heart

An Efficient Transgenesis Approach for Gene Delivery in the Mouse Embryonic Heart

Published on: May 24, 2024

In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells

In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells

Published on: August 9, 2017

Related Experiment Videos

Last Updated: Jun 2, 2026

Modified Mouse Embryonic Stem Cell based Assay for Quantifying Cardiogenic Induction Efficiency

Modified Mouse Embryonic Stem Cell based Assay for Quantifying Cardiogenic Induction Efficiency

Published on: April 22, 2011

An Efficient Transgenesis Approach for Gene Delivery in the Mouse Embryonic Heart

An Efficient Transgenesis Approach for Gene Delivery in the Mouse Embryonic Heart

Published on: May 24, 2024

In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells

In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells

Published on: August 9, 2017

Main Results:

Successfully identified crucial time windows for Wnt/β-catenin and BMP signal activation.
Demonstrated rapid quantification of cardiogenic efficiency via EB contraction scoring.
Validated the assay's applicability beyond cardiac differentiation to other cell lineages.

Conclusions:

The developed 96-well assay provides a quantitative and efficient method for studying signaling pathway roles in stem cell differentiation.
This platform facilitates the identification of key temporal signaling requirements for specific cell fate commitments.
The assay has potential for high-throughput screening and automated analysis in developmental and regenerative medicine research.