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Related Experiment Video

Updated: Jun 2, 2026

Associated Chromosome Trap for Identifying Long-range DNA Interactions
14:49

Associated Chromosome Trap for Identifying Long-range DNA Interactions

Published on: April 23, 2011

Associated chromosome trap for identifying long-range DNA interactions.

Jianqun Ling1, Andrew R Hoffman

  • 1Medical Service, VA Palo Alto Health Care System, USA.

Journal of Visualized Experiments : Jove
|May 5, 2011
PubMed
Summary

The Associated Chromosome Trap (ACT) assay identifies long-range DNA associations, revealing how nuclear architecture impacts gene expression. This method aids in understanding transcriptional regulation in normal and diseased states.

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Area of Science:

  • Genetics
  • Molecular Biology
  • Cell Biology

Background:

  • DNA is organized into chromatin, with chromosomes occupying distinct territories.
  • Gene expression involves looping of chromatin segments, potentially bringing distant DNA regions together.
  • Understanding nuclear architecture's role in gene regulation is crucial for physiology and disease.

Purpose of the Study:

  • To introduce and validate the Associated Chromosome Trap (ACT) assay.
  • To investigate mechanisms of long-range DNA associations and their role in gene expression.
  • To explore the link between nuclear architecture and gene regulation in health and disease.

Main Methods:

  • The Associated Chromosome Trap (ACT) assay, an extension of chromosome conformation capture.

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Last Updated: Jun 2, 2026

Associated Chromosome Trap for Identifying Long-range DNA Interactions
14:49

Associated Chromosome Trap for Identifying Long-range DNA Interactions

Published on: April 23, 2011

RNA-Associated Chromatin DNA-DNA Interaction Method
11:01

RNA-Associated Chromatin DNA-DNA Interaction Method

Published on: April 30, 2026

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Capturing Chromosome Conformation Across Length Scales

Published on: January 20, 2023

  • Unbiased identification of long-range DNA associations within the nucleus.
  • Investigating transcriptional regulation in trans.

    • Main Results:

    • The ACT assay effectively identifies long-range DNA associations.
    • Demonstrates the potential for chromatin segments from different chromosomes or distant regions to co-localize.
    • Provides a method to study nuclear organization's impact on gene expression.

    Conclusions:

    • The ACT assay is a powerful tool for studying nuclear architecture and gene expression.
    • Facilitates research into mechanisms of transcriptional regulation.
    • Offers insights into the relationship between nuclear organization and disease states.