Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Reversibly-sealable microfluidic platform for multi-molecule gradient delivery to large adherent cell cultures.

Biomedical microdevices·2026

Same author

Bepirovirsen induces innate immune activation in the liver potentially through TLR8 signaling.

JHEP reports : innovation in hepatology·2026

Same author

Immunomodulation by bepirovirsen may induce killing of infected hepatocytes (B-Together study).

Hepatology international·2025

Same author

Combination Therapy With Olaparib Plus Lenvatinib in a Patient With <i>BRCA2</i>-Mutated, Radioiodine-Refractory, Metastatic Papillary Thyroid Cancer: A Case Report.

JCO precision oncology·2025

Same author

Viral Nervous Necrosis due to Betanodavirus: A Case Study in Pot-Bellied Seahorses (Hippocampus abdominals).

Journal of fish diseases·2025

Same author

Functionally distinct GABAergic amacrine cell types regulate spatiotemporal encoding in the mouse retina.

Nature neuroscience·2025

Same journal

A Video Protocol of a Randomized Controlled Clinical Trial - Electrochemotherapy of Cutaneous Metastases with Reduced Dose Bleomycin (BLESS Trial).

Journal of visualized experiments : JoVE·2026

Same journal

A Standardized Ex Vivo Porcine Oromucosal Model for Evaluating Peptide Fluxes.

Journal of visualized experiments : JoVE·2026

Same journal

Lightweight English Text Classification with Deep Learning Based on Complex System Theory.

Journal of visualized experiments : JoVE·2026

Same journal

Integrating Artificial Intelligence-Assisted Translation Support into English Courses: Effects on Translation Accuracy, Perceived Stress, and Anxiety.

Journal of visualized experiments : JoVE·2026

Same journal

A Toxin-Based Counter-Selection System for Markerless Gene Deletion and High-Density Tn5 Transposon Mutagenesis in Pectobacterium brasiliense.

Journal of visualized experiments : JoVE·2026

Same journal

Seamless Multimodal Human-Robot Communication: Integration Techniques in Human-Computer Interaction.

Journal of visualized experiments : JoVE·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 2, 2026

Transcriptome Analysis of Single Cells

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

Transcriptome analysis of single cells.

Jacqueline Morris¹, Jennifer M Singh, James H Eberwine

¹Department of Pharmacology, University of Pennsylvania, USA.

Journal of Visualized Experiments : Jove

|May 5, 2011

Summary

This summary is machine-generated.

Researchers developed a simple method to isolate single cells for gene expression analysis. This technique, coupled with antisense RNA amplification, offers a more accurate transcriptome analysis than traditional PCR methods.

More Related Videos

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Published on: January 7, 2020

Related Experiment Videos

Last Updated: Jun 2, 2026

Transcriptome Analysis of Single Cells

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Published on: January 7, 2020

Area of Science:

Neuroscience
Molecular Biology
Genomics

Background:

Gene expression analysis often uses heterogeneous cell populations, limiting detailed study of specific cell types.
Brain regions like the hippocampus contain diverse cells with unique mRNA profiles, necessitating single-cell analysis.

Purpose of the Study:

To present a rapid method for isolating single cells from brain tissue or cell cultures for molecular analysis.
To describe antisense RNA (aRNA) amplification as a downstream application for accurate gene expression profiling of single cells.

Main Methods:

Utilizing electrophysiology pipettes for single-cell aspiration and isolation.
Implementing a two-round antisense RNA amplification protocol involving T7 RNA polymerase and in vitro transcription.
Adapting the method for harvesting dendrites or cells from acute brain slices.

Main Results:

The single-cell isolation method is simple, rapid, and adaptable to various biological samples.
Antisense RNA amplification provides linear amplification from minimal starting material (femtograms to micrograms).
Linear amplification yields more accurate transcriptome abundance estimations compared to exponential PCR amplification.

Conclusions:

Single-cell isolation combined with aRNA amplification is a powerful approach for detailed molecular investigation of cell populations.
This method overcomes limitations of bulk tissue analysis, enabling precise study of cellular heterogeneity.
The protocol facilitates accurate gene expression profiling, crucial for understanding complex biological systems like the brain.