Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
Proton (¹H) NMR: Chemical Shift01:07

Proton (¹H) NMR: Chemical Shift

Organic molecules primarily contain carbon and hydrogen atoms. While all the hydrogen isotopes are NMR-active, protium or hydrogen-1 is the most abundant. It has a significant energy separation between its nuclear spin states due to its large gyromagnetic ratio. As per Boltzmann's distribution, an increase in the energy separation implies a greater excess population of nuclei available for excitation, resulting in a strong NMR absorption signal.
Absorption signals of all the protium nuclei in a...
¹H NMR of Labile Protons: Temporal Resolution01:10

¹H NMR of Labile Protons: Temporal Resolution

Protons bonded to heteroatoms such as nitrogen and oxygen exhibit a range of chemical shift values. This is due to the varying degree of hydrogen bonding between the proton and the heteroatom in other molecules. The extent of hydrogen bonding affects the electron density around the proton, thereby giving different chemical shift values for the protons in the proton NMR spectrum.
The –OH proton in alcohols typically appears in the range of δ 2 to 5 ppm but can vary depending on the specific...
¹H NMR of Labile Protons: Deuterium (²H) Substitution00:48

¹H NMR of Labile Protons: Deuterium (²H) Substitution

This lesson illustrates the role of deuterium substitution in simplifying the NMR spectrum of compounds comprising labile protons. One method employed is the use of deuterium. Amongst the three isotopes of hydrogen, deuterium (2H) has a nucleus composed of one proton and one neutron. When the D2O solvent is added to a pure dry ethanol solution, its labile proton is substituted with deuterium.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Deciphering resistance mechanisms to auxin-inducible protein degradation in mammalian cells.

The Journal of biological chemistry·2026
Same author

Alternative organelle targeting of OPA1 mediates fatty acid release from lipid droplets.

bioRxiv : the preprint server for biology·2026
Same author

Elevated endocytic trafficking mediated by GPRASP2 maintains HSC fidelity.

bioRxiv : the preprint server for biology·2026
Same author

Deciphering acquired resistance mechanisms to sustained auxin-inducible protein degradation in cells and mice.

bioRxiv : the preprint server for biology·2025
Same author

Rapid and robust validation of pooled CRISPR knockout screens using CelFi.

Scientific reports·2025
Same author

Modeling GATA2 deficiency in mice: the R396Q mutation disrupts normal hematopoiesis.

Leukemia·2025

Related Experiment Video

Updated: Jun 2, 2026

Methodology for Accurate Detection of Mitochondrial DNA Methylation
12:11

Methodology for Accurate Detection of Mitochondrial DNA Methylation

Published on: May 20, 2018

Detecting DNA methylation through changes in transverse proton relaxation.

Hamsa Jaganathan1, Pengfei Wang, Jonathon Klein

  • 1Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, USA.

The Analyst
|May 5, 2011
PubMed
Summary

We developed a simple method to detect DNA methylation using superparamagnetic nanoparticles and NMR. This technique measures changes in proton relaxation behavior to identify methylation in DNA strands.

More Related Videos

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
06:07

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

Related Experiment Videos

Last Updated: Jun 2, 2026

Methodology for Accurate Detection of Mitochondrial DNA Methylation
12:11

Methodology for Accurate Detection of Mitochondrial DNA Methylation

Published on: May 20, 2018

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
06:07

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

Area of Science:

  • Biochemistry
  • Nanotechnology
  • Molecular Biology

Background:

  • DNA methylation is a crucial epigenetic modification regulating gene expression.
  • Accurate detection of DNA methylation is vital for understanding various biological processes and diseases.
  • Current methods for DNA methylation detection can be complex and time-consuming.

Purpose of the Study:

  • To present a facile and simple method for detecting DNA methylation.
  • To utilize the transverse proton relaxation behavior for methylation analysis.
  • To explore the application of superparamagnetic nanoparticles (NPs) in NMR-based DNA methylation detection.

Main Methods:

  • Positively charged superparamagnetic nanoparticles (NPs) were electrostatically arranged along negatively charged DNA backbones.
  • The transverse proton relaxation signal was measured for both uncut and enzyme-cleaved DNA strands.
  • Sequence-specific restriction enzymes were used to cleave DNA strands at specific sites.

Main Results:

  • The arrangement of NPs along DNA amplified the transverse proton relaxation signal.
  • A difference in relaxation signals was observed between cut and uncut DNA strands, indicating enzyme activity.
  • The presence of DNA methylation was successfully detected by analyzing the transverse proton relaxation behavior using superparamagnetic NPs and NMR.

Conclusions:

  • This study demonstrates a straightforward and effective method for DNA methylation detection.
  • Superparamagnetic nanoparticles combined with NMR offer a sensitive approach for epigenetic analysis.
  • The developed technique has potential applications in molecular diagnostics and biological research.