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Multipotency and Niche of Bulge Stem Cell01:06

Multipotency and Niche of Bulge Stem Cell

A hair follicle or HF is a small part of the skin that produces the hair shaft. Paul Gerson Unna was the first to observe a bulge in the human hair follicle's outer root sheath (ORS). The bulge is present between the sebaceous gland and the arrector pili muscle and is the niche for hair follicle stem cells (HFSCs). The bulge is also a niche for melanocyte stem cells, and their loss results in graying of hair. The HFSCs express Sox9 and Lhx2, which help them maintain stemness and prevent...

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Mammosphere Assay Reveals Api5-Induced Stemness in Non-Tumorigenic Breast Epithelial Cell Lines
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Multipotent human breast stem cell line MCF1OAT.

L Tait1, P Dawson, S Wolman

  • 1BARBARA ANN KARMANOS CANC INST,BREAST CANC PROGRAM,DETROIT,MI 48201. WAYNE STATE UNIV,SCH MED,DEPT PATHOL,DETROIT,MI 48201. UNIV S FLORIDA,DEPT PATHOL & LAB MED,TAMPA,FL 33612. JAMES A HALEY VET HOSP,TAMPA,FL 33612. GEORGE WASHINGTON UNIV,DEPT PATHOL,WASHINGTON,DC.

International Journal of Oncology
|May 5, 2011
PubMed
Summary
This summary is machine-generated.

Human breast epithelial cells form ducts in mice that mimic human breast disease and cancer. These ducts feature a bilayer structure with a basement membrane, confirming their human origin and myoepithelial characteristics.

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Area of Science:

  • Biomedical research
  • Cell biology
  • Cancer research

Background:

  • Human breast epithelial MCF10AT cells can form ducts in vivo.
  • These structures can model human proliferative breast disease and cancer progression.

Purpose of the Study:

  • To characterize the structure and cellular composition of ducts formed by human breast epithelial cells in mice.
  • To confirm the human origin and myoepithelial nature of these in situ formed ducts.

Main Methods:

  • In vivo injection of MCF10AT cells with Matrigel into nude/beige mice.
  • Silver staining for basement membrane visualization.
  • Immunohistochemistry for smooth muscle actin to detect myoepithelial cells.
  • Fluorescent in situ hybridization for human chromosome 9 to confirm human origin.
  • Electron microscopy to study cell junctions and ultrastructure.

Main Results:

  • MCF10AT cells formed bilayered ducts with luminal and myoepithelial layers, resembling human breast disease.
  • A distinct basement membrane surrounded the ducts.
  • Human origin of both cell layers was confirmed.
  • Ultrastructural analysis revealed characteristic myoepithelial features like actin bundles and hemidesmosomes, and cell-cell junctions.

Conclusions:

  • The in vivo model using MCF10AT cells successfully recapitulates key features of human breast ducts and disease.
  • This model provides a valuable tool for studying breast cancer development and progression.